FastQCFastQC Report
Fri 7 Jul 2023
SRR7235654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22042880
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length83
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3512501.5934850618430985No Hit
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2588651.1743701367516406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2482661.1262865832413913Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1859940.8437826636083852Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1221030.5539339687010046Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC934290.42385114830729925Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA897360.4070974391731026Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC852960.3869548806689507Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC834080.37838975669241043Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA828550.37588101010394287Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA667740.3029277480982522Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA597760.2711805353928343Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA540400.24515852737936242Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA495930.22498421258928056Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA467370.2120276479298531No Hit
GCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC420420.19072825329539517Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA417490.18939902589861216Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC400980.18190907903141512Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA349880.1587269903025376No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA298120.1352454851634632No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG285710.12961554932930724No Hit
ATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA284790.12919818099994193No Hit
GGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA283080.12842242030079554Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA281450.12768295249985484Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281310.1276194399279949No Hit
CGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC279950.12700246065849835Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278310.12625845624528192No Hit
GGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC276280.1253375239533128Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA259200.11758899018640033Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA255400.11586507752163057Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251790.11422736049009928No Hit
GGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA241020.10934142906915975Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA240400.10906015910806574Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC238520.10820727600023226Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA234560.10641077753905116Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA228600.10370695662272807No Hit
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCT224600.10189231171244412TruSeq Adapter, Index 1 (97% over 36bp)
GGGAAAAAAAAAAAAAAAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA223470.10137967452528888Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCCCCCGTCTGAACTCCAGTCA221490.10048142529469833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)