FastQCFastQC Report
Tue 15 Aug 2023
SRR7235815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43387110
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA41624149.593665030927388No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC35593808.203772963905639No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG8235981.898255034732666TruSeq Adapter, Index 5 (100% over 49bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT7907211.8224790727015465Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA6780851.5628720142918024No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6738291.553062649252278Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5272331.215183495743321No Hit
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC4617351.0642216086759408Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG4229810.9749001489151963No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT3992420.9201857418021159No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC3928110.9053633671383045No Hit
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3442730.7934914309803074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG3328900.7672555281971996No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3085500.7111559170454083No Hit
ACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC3052530.7035568859045924Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA2830170.6523066413042953No Hit
ATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCA2614010.6024853925509212Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG2473620.5701278559461554No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC2123730.4894840887074525No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2002200.4614734652757467No Hit
ACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1861510.42904678371064586Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1767270.40732604683741325No Hit
TCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1525050.35149840586293946Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT1447420.33360599496025434Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1199140.2763816257870137No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1190470.27438333643333235Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1155840.26640170317866296Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTC1144410.2637672801898997No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1141310.26305278226643813No Hit
AACCTCGGGCCGATCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1070040.24662624452285484Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAG1008580.23246074698222582Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA985770.22720342516475514Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC950060.21897287005287974TruSeq Adapter, Index 5 (100% over 35bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG873800.2013962211357244No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC846390.19507867659311717No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG814420.18771012865341802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA732790.1688957849462663No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG687650.15849177324786093No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACATGAGATCGGAAG675620.1557190603384277No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG671000.1546542279492688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC645730.14882991745705118No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACAGATCGGAAGAGC573710.1322305173126304No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC565390.1303128970793399Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT560570.1292019680499577No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCACACAGATCGGAAGAGCACA548660.12645691312465845No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC547440.12617572361929616TruSeq Adapter, Index 5 (100% over 50bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC541230.12474442294036177No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA521070.12009788160585022No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA515350.11877951769546302No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT511810.11796360716351008No Hit
ACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA510170.11758561471367879Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT493330.11370427760687449No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC484200.11159996598067952No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG474970.10947260603437288TruSeq Adapter, Index 5 (100% over 50bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA466280.10746970701666925No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCACACAGATCGGAAGAGCAC452910.10438814661773971No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC446370.10288078648243684Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC442500.10198881649411541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)