FastQCFastQC Report
Tue 22 Aug 2023
SRR7235816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53565229
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG41033357.660445174237937TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG836950.15624874860518193TruSeq Adapter, Index 1 (97% over 49bp)
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG687900.12842286177848694No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC587220.10962708663114275TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC575970.10752684357981555TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)