FastQCFastQC Report
Tue 22 Aug 2023
SRR7235817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42636081
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA40790289.567080051283325No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC35092538.230711917448511No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT7637721.791374774806343Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG7273251.7058908392635808TruSeq Adapter, Index 5 (100% over 49bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6760201.585558485077463Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA6585451.5445720726536756No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5258381.2333169176594818No Hit
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC4669231.0951358310816606Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG4228210.9916976187375195No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT3967020.93043729793083No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC3853730.90386590643732No Hit
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3525120.8267926876299911Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG3301310.774299589120304No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3082640.7230120423122379No Hit
ACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2997290.7029937859438817Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA2831150.6640267898918759No Hit
ATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCA2527810.592880476045629Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG2410540.565375602884327No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC2103150.4932793893510053No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1957740.4591744724380273No Hit
ACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1882610.4415532468849564Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1773490.41595990025443474No Hit
TCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1496790.3510618154609473Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT1377290.3230339111139225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1181890.27720418300171634No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1155900.2711084069851542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1118950.2624420382351746No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTC1116330.2618275352277335No Hit
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1114340.26136079439383747Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCTCGGGCCGATCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1039990.2439225124842032Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA996190.23364952327583766Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAG971470.2278516170376916Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC934630.2192110480322992TruSeq Adapter, Index 5 (100% over 35bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG864890.20285400996400207No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC850140.19939449875798856No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG802250.18816222813724368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA735500.1725064740354537No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG683880.16039935753006943No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACATGAGATCGGAAG680300.15955969311532173No Hit
ACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTG651460.15279546917081804Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC642560.15070803529057936No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACAGATCGGAAGAGC572320.1342337256559767No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG551450.12933881047838333TruSeq Adapter, Index 5 (97% over 49bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT550980.12922857520605613No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC545050.1278377344296724Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCACACAGATCGGAAGAGCACA538560.12631554949902643No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC531080.1245611668670955No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA521030.12220400838435408No Hit
ACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA510580.11975303264856825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA504280.1182754109131184No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT491790.11534596718680594No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC476100.111665985436138No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT474760.11135169763843915No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA451740.10595251472573193No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCACACAGATCGGAAGAGCAC446930.10482436225787262No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC437610.10263842026193731Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC428590.10052284120578531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)