FastQCFastQC Report
Tue 22 Aug 2023
SRR7235819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59600521
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT41237286.918946228674747No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC34402775.772226387081416No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG31939555.358938053578425No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC20296603.4054400296265865No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA16724362.8060761415156086No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG12827452.152237897383481TruSeq Adapter, Index 6 (100% over 49bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA10391991.7436072412856929No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG10307841.7294882371917522No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT8370531.4044390652222654No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA8036651.3484194206960036No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC5954010.9989862336941652No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA5103300.8562509042496457No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA4311720.7234366290187295No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT4243010.7119082063057804No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC4157700.6975945730407289No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT4065180.6820712188069632No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3334150.5594162507404927No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA2394520.4017615886277236No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2286350.38361241842164434No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGA2127250.3569180209011931No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2098050.35201873486978413No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA1951490.3274283458025476No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAG1939770.32546192003925606No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG1867450.3133277979231088No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1835110.30790167085955505No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC1738160.2916350345326679Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1708560.2866686349939794No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1684280.2825948451021091No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1572540.2638466868435596No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG1543880.2590380040469781No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATAGATCGGAAGAGCA1459530.2448854431993975No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA1440700.24172607484421152No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC1374100.23055167588216216No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1348300.22622285466262954No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA1293740.21706857226969542No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1282120.2151189248832238No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGC1165340.19552513643295166No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1163540.19522312565019356No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1125840.18889767758909357No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1015510.17038609444370462No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC1010390.16952704155052603No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC993970.16677203207669947No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC988760.165897878644383No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG946780.15885431605539152No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT919080.15420670567628092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGC911590.15295000525247085No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG903630.1516144464576073No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC866410.1453695346052428No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC849850.14259103540386836No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA840770.141067558788622No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC820550.13767497099563944No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA814880.13672363702995147No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAG799940.1342169475330593No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG798600.13399211728367275No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG754060.126519028248092No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC734230.12319187612470704No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT732550.12290999939413282No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC724060.12148551520212382No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT721730.12109457902222029No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAA698900.1172640755942385No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT698000.11711307020285947No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC694010.11644361296774569Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAGATCGGA677760.11371712673451295No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA631680.10598565069590583Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTC608800.1021467580795141No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCA604280.1013883754472549No Hit
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT601520.10092529224702583Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG597530.10025583501191206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)