FastQCFastQC Report
Tue 22 Aug 2023
SRR7235821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7235821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58834836
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT41031606.974031507455888No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC33917065.764792137773615No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG31526655.358500531895763No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC20180963.4301038928705436No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA16382752.784532279481496No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG12428632.1124610596348057TruSeq Adapter, Index 6 (100% over 49bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG10283851.7479185290836876No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA10138241.7231695861275111No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT8340101.4175445309306207No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA7917461.3457095384781899No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC6003751.0204413589255181No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA4998310.8495494064094953No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA4357380.7406122454390797No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC4105750.6978433661309092No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT4102370.6972688765546997No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT4002030.6802143546384662No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3314820.5634111056245658No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA2359240.4009937241942852No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2300640.3910336386422493No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2112490.3590542854576836No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGA2066150.35117799937438426No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAG1929620.3279723597767826No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA1905000.3237877641062856No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1859300.31602025711433956No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG1849590.3143698743377138No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1705350.28985378662396544No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC1680600.2856470952005373Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1662260.28252989436394454No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1566260.2662130306609506No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG1557970.264804001493265No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATAGATCGGAAGAGCA1473520.2504502604545375No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA1436510.2441597695623729No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1368150.2325408028671993No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC1354860.2302819370483161No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1295700.22022666979134606No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA1271310.21608116660680418No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1143790.19440693265466058No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGC1141480.19401430812180728No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1113450.18925012385519355No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC1014690.17246415032073856No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1013470.17225679017784631No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC975220.16575553979618468No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC970630.1649753897503853No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG944870.16059703132341527No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT929270.15794554097167873Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGC915900.15567307776637637No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG881500.14982620160613688No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC853670.14509601080557105No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC829960.141066085405592No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC822800.13984911932107705No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA822710.1398338222613555No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA814510.13844009015339143No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAG786970.13375918987859506No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG774890.13170598452930166No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG738310.12548857958914No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT737770.12539679723081068No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC732590.12451636646016995No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT715940.12168641041168196No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC712210.12105243226988853No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT702380.11938165341363405No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAA684620.11636303362858019No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC671460.1141262635626281Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTAGATCGGA663880.11283791119941254No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA628640.10684826248177186Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG598930.10179853310035572No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCA598480.10172204780174793No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTC594920.10111696410609525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)