FastQCFastQC Report
Fri 7 Jul 2023
SRR7252574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7252574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42309934
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN683091816.144950734264913No Hit
TAGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNN3518520.8316061187899749No Hit
AGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNNN2666430.6302136987498019No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGANN2456600.5806201446686257No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATAGANNNNN2265400.5354298118262251No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGANNNNN2177080.5145552815090659No Hit
GAGCGCTATGTGGCATATAGCTCGAGAGANNNNNN2034110.4807641628559383No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNN1931010.45639636308579445No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGN1763900.4168997285602005No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAAN1745180.4124752357212375No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGAN1586590.37499231268004346No Hit
AACGTCTATCTGAGGGTCACTAGANNNNNNNNNNN1584290.37444870511970074No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGANNNNNN1562500.3692986143632368No Hit
ATTGCGATCCGTTGAAACTCAGANNNNNNNNNNNN1545540.36529009948349245No Hit
TTTTTGGAGCAGAGCGCTATGTGGCAGANNNNNNN1505550.3558384184669255No Hit
AAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNNN1401590.3312673567394362No Hit
ATCCGGCGCGCTTGTGGCGAGANNNNNNNNNNNNN1164670.2752710510018758No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGANNNNN1150010.2718061436824742No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGANN1115810.2637229356112917No Hit
AAATGAATCCAGAGACGTTGGCACAGANNNNNNNN1094840.25876665276764554No Hit
GTGATGGCCGCATCGGCGACTCAGGAGANNNNNNN1030550.24357163970050152No Hit
AGTGATGGCCGCATCGGCGACTCAGGAGANNNNNN1009160.23851608938931457No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGAGANNNNNNN960150.22693252133175157No Hit
AATGAATCCAGAGACGTTGGCACAGANNNNNNNNN893850.2112624425270907No Hit
TCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNN885980.20940235926626594No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATATAGANNN846590.2000924889176145No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAGN841830.1989674576188183No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAN836710.19775733991927286No Hit
AATATTCCCGCACTTTGTGAGANNNNNNNNNNNNN832130.19667485182085134No Hit
TGAGTCTGAATATTCGTGCATGGAATAAGANNNNN824550.1948833103828524No Hit
GACTTGGGCCAACGTCTATCAGANNNNNNNNNNNN769010.18175636955614255No Hit
TGGGAATACCTCGTGCGCTAGANNNNNNNNNNNNN752700.17790148289997332No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGANNNNNNN752250.17779512489903673No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTGAGANNN709620.16771947694364167No Hit
TAGAGATAAATCGATGCCAAAGAGANNNNNNNNNN708860.16753985009761538No Hit
CTTGGGCCAACGTCTATCTGAGGGTCACTAGANNN700950.16567031279226294No Hit
TCCTTCGGGGGAGTGTTATAGANNNNNNNNNNNNN676280.15983953082980465No Hit
GACTTGGGCCAACGTCTATAGANNNNNNNNNNNNN673940.15928646922493428No Hit
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGAN665970.15740275085279026No Hit
ATACCGCGTAGAATAAACCAGTTCTAGANNNNNNN638600.15093382088471233No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGANNNNNNN632240.1494306278048082No Hit
GACTTGGGCCAACGTCTATCTGAGAGANNNNNNNN620850.14673858862554595No Hit
TAAACTTATACTCAGCCGTCAGTGTAAGANNNNNN615490.1454717466588343No Hit
GACTTGGGCCAACGTCTATCTGAAGANNNNNNNNN615380.14544574803638313No Hit
AGCTGGTAGAGATAAATCGATGCCAGANNNNNNNN612040.14465633531832028No Hit
CAAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNN605550.14312241659370115No Hit
TTGAATCAACTGTAGACGACTTTAAGCAGANNNNN580080.1371025537406889No Hit
TTGTGACATCCGGCGCGCTTGTGGCAGANNNNNNN573990.13566317546134674No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTAGANNNN572290.13526137856891954No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTTAGANNNNN559670.13227862752043054No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTAGANNNNNN555790.13136158520124375No Hit
TGAGTCTGAATATTCGTGCATGGAATAGANNNNNN553360.13078725199618604No Hit
AAAGCGAGAGCTTAGAGTCGAGAGANNNNNNNNNN542910.12831738286332472No Hit
CAAATGAATCCAGAGACGTTGGCACAGANNNNNNN533400.12606968377686434No Hit
CGGCGATGGATCATTCAAGTGTCTGACCTATCAGN532040.12574824626292255No Hit
GAGCAGAGCGCTATGTGGCATAGANNNNNNNNNNN529960.1252566359474822No Hit
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAN529580.1251668225244691No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGANNNNNN529280.12509591719051133No Hit
AGAGCGCTATGTGGCATATAGCTCGAGAGANNNNN516250.12201626218561344No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGANNN510730.120711604040791No Hit
AAACTTATACTCAGCCGTCAGTGTAAGANNNNNNN507940.120052184434984No Hit
AAATGAATCCAGAGACGTTGGCAGANNNNNNNNNN497630.1176154044579696No Hit
AGCGCTATGTGGCATATAGCTCGAGAGANNNNNNN493020.11652582582615233No Hit
TGACCTCAGATTAGTCGAGAGCAGANNNNNNNNNN492520.11640765026955607No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGANNN488810.11553078763961201No Hit
AGAGCGCTATGTGGCATATAGANNNNNNNNNNNNN482950.11414577011630413No Hit
TCGTGCGAGTTTAAGTACATTTGAATGTGTCTAGN459270.10854897575590641No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAATAGANNNNN458400.10834335028742895No Hit
CGTGTGGTAACACAAATGAATCCAGAGACGTTGGCAC458010.10825117335328388No Hit
CGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNNN456960.1080030046844318No Hit
CGTGTGGTAACACAAATGAATCCAGAGACGTTGGCA446760.10559222332986859No Hit
TTCTGTGTCGCGGAAGAGCGTGCGTGAGANNNNNN439890.10396849118223629No Hit
AACTGTAGACGACTTTAAGCGAGANNNNNNNNNNN439100.10378177380281424No Hit
ATGAATCCAGAGACGTTGGCACAGANNNNNNNNNN438220.10357378482320487No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGANN437650.10343906468868516No Hit
AAATGAATCCAGAGACGTTGGCACAAGANNNNNNN437120.10331379859869316No Hit
AAAGCGAGAGCTTAGAGTCGAGTAGANNNNNNNNN432780.10228803476743784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)