FastQCFastQC Report
Fri 7 Jul 2023
SRR7252575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7252575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88149559
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1390809915.777842972532625No Hit
TAGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNN7240250.8213597529171983No Hit
AGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNNN5288780.5999780441329263No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGANN5124710.5813653588442796No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATAGANNNNN4673080.5301308427419359No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGANNNNN4484060.5086877405705456No Hit
GAGCGCTATGTGGCATATAGCTCGAGAGANNNNNN4249560.4820852251796291No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNN3998800.45363811746352584No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGN3657240.4148903342783598No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAAN3509340.3981120313942807No Hit
AACGTCTATCTGAGGGTCACTAGANNNNNNNNNNN3328940.3776468127310767No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGAN3229620.3663795981100711No Hit
ATTGCGATCCGTTGAAACTCAGANNNNNNNNNNNN3205130.3636013652660474No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGANNNNNN3119230.35385656325291426No Hit
TTTTTGGAGCAGAGCGCTATGTGGCAGANNNNNNN3087790.3502898976499701No Hit
AAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNNN2876500.326320407343161No Hit
ATCCGGCGCGCTTGTGGCGAGANNNNNNNNNNNNN2410410.2734454973280127No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGANNNNN2338050.2652367211502442No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGANN2335120.2649043315123108No Hit
AAATGAATCCAGAGACGTTGGCACAGANNNNNNNN2244760.25465357121071924No Hit
GTGATGGCCGCATCGGCGACTCAGGAGANNNNNNN2150450.24395470883751105No Hit
AGTGATGGCCGCATCGGCGACTCAGGAGANNNNNN2094220.23757577732181281No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGAGANNNNNNN1990600.22582075538233834No Hit
TCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNN1868170.2119318600334688No Hit
AATGAATCCAGAGACGTTGGCACAGANNNNNNNNN1844990.2093022382562345No Hit
AATATTCCCGCACTTTGTGAGANNNNNNNNNNNNN1751070.19864761887237575No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATATAGANNN1739000.19727835507379No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAN1726670.19587959594897122No Hit
TGAGTCTGAATATTCGTGCATGGAATAAGANNNNN1704570.1933724932191663No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAGN1692690.1920247836974431No Hit
TGGGAATACCTCGTGCGCTAGANNNNNNNNNNNNN1565010.17754030964579187No Hit
GACTTGGGCCAACGTCTATCAGANNNNNNNNNNNN1561070.17709334200979948No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGANNNNNNN1553930.17628335497401637No Hit
TAGAGATAAATCGATGCCAAAGAGANNNNNNNNNN1504090.17062932782227533No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTGAGANNN1472260.16701841922998162No Hit
CTTGGGCCAACGTCTATCTGAGGGTCACTAGANNN1460180.16564802099577153No Hit
TCCTTCGGGGGAGTGTTATAGANNNNNNNNNNNNN1417050.1607552001479667No Hit
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGAN1410520.1600144136852687No Hit
GACTTGGGCCAACGTCTATAGANNNNNNNNNNNNN1374980.1559826294763426No Hit
ATACCGCGTAGAATAAACCAGTTCTAGANNNNNNN1330620.1509502730467432No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGANNNNNNN1326480.1504806166982639No Hit
TAAACTTATACTCAGCCGTCAGTGTAAGANNNNNN1301150.1476070912617952No Hit
GACTTGGGCCAACGTCTATCTGAGAGANNNNNNNN1271630.14425823729872544No Hit
CAAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNN1266340.14365812085344634No Hit
GACTTGGGCCAACGTCTATCTGAAGANNNNNNNNN1258820.14280502526393807No Hit
AGCTGGTAGAGATAAATCGATGCCAGANNNNNNNN1251980.14202907129688533No Hit
TTGAATCAACTGTAGACGACTTTAAGCAGANNNNN1227180.13921567094850695No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTAGANNNN1174640.13325534617819246No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTAGANNNNNN1153000.13080042748710743No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTTAGANNNNN1148100.13024455403117785No Hit
TTGTGACATCCGGCGCGCTTGTGGCAGANNNNNNN1147130.13013451377561627No Hit
TGAGTCTGAATATTCGTGCATGGAATAGANNNNNN1139510.12927007382986455No Hit
CGGCGATGGATCATTCAAGTGTCTGACCTATCAGN1137000.12898533048815367No Hit
AAAGCGAGAGCTTAGAGTCGAGAGANNNNNNNNNN1121290.1272031321223059No Hit
CAAATGAATCCAGAGACGTTGGCACAGANNNNNNN1120900.12715888913295642No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGANNNNNN1120460.12710897396548518No Hit
GAGCAGAGCGCTATGTGGCATAGANNNNNNNNNNN1112920.1262536095047282No Hit
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAN1110240.12594958075740345No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGANNN1085170.12310555064716773No Hit
AAACTTATACTCAGCCGTCAGTGTAAGANNNNNNN1068830.12125188283698618No Hit
AGAGCGCTATGTGGCATATAGCTCGAGAGANNNNN1042070.11821613310623595No Hit
AGCGCTATGTGGCATATAGCTCGAGAGANNNNNNN1028180.1166404020240192No Hit
TGACCTCAGATTAGTCGAGAGCAGANNNNNNNNNN1024910.11626944157485802No Hit
AAATGAATCCAGAGACGTTGGCAGANNNNNNNNNN1024390.11621045092239202No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGANNN1011150.11470845815575775No Hit
AGAGCGCTATGTGGCATATAGANNNNNNNNNNNNN969670.11000281918597007No Hit
TCGTGCGAGTTTAAGTACATTTGAATGTGTCTAGN968520.10987235908917026No Hit
CGTGTGGTAACACAAATGAATCCAGAGACGTTGGCAC958840.10877422540480322No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAATAGANNNNN950000.10777138431288125No Hit
CGTGTGGTAACACAAATGAATCCAGAGACGTTGGCA940420.10668459498475767No Hit
CGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNNN924040.10482638943207871No Hit
ATGAATCCAGAGACGTTGGCACAGANNNNNNNNNN919450.10430568348050386No Hit
TTCTGTGTCGCGGAAGAGCGTGCGTGAGANNNNNN917610.10409694732562418No Hit
AACTGTAGACGACTTTAAGCGAGANNNNNNNNNNN905510.10272428022016537No Hit
CTTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNN894100.10142988917278643No Hit
AAATGAATCCAGAGACGTTGGCACAAGANNNNNNN890880.101064600901747No Hit
AAAGCGAGAGCTTAGAGTCGAGTAGANNNNNNNNN889180.10087174684560815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)