FastQCFastQC Report
Fri 7 Jul 2023
SRR7252576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7252576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44631407
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1395826931.27454395511215No Hit
AGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNNN9776582.190515750489336No Hit
TAGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNN8008061.794265638992739No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATAGANNNNN2974880.6665440773579018No Hit
ATCCGGCGCGCTTGTGGCGAGANNNNNNNNNNNNN2906700.6512678392594703No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGANNNNNN2107400.47217870590546246No Hit
TTTTTGGAGCAGAGCGCTATGTGGCAGANNNNNNN1982660.4442297774748621No Hit
ATTGCGATCCGTTGAAACTCAGANNNNNNNNNNNN1582330.35453285172031435No Hit
TCCTTCGGGGGAGTGTTATAGANNNNNNNNNNNNN1549910.3472689086409487No Hit
AACGTCTATCTGAGGGTCACTAGANNNNNNNNNNN1359590.3046262915260547No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNN1272260.2850593529350307No Hit
AGAGCGCTATGTGGCATATAGANNNNNNNNNNNNN1150270.2577265825386146No Hit
AAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNNN1122200.251437289440595No Hit
AAATGAATCCAGAGACGTTGGCACAGANNNNNNNN1100590.2465954075792412No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGANNNNN1042820.23365160771203114No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATATAGANNN1017020.22787092506404738No Hit
TGGGAATACCTCGTGCGCTAGANNNNNNNNNNNNN1010480.2264055892300236No Hit
TGAGTCTGAATATTCGTGCATGGAATAAGANNNNN997530.22350404503268295No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGN988960.2215838725406976No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTGAGANNN988270.2214292728884841No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGANNNNN973640.21815131214662353No Hit
TTGTGACATCCGGCGCGCTTGTGGCAGANNNNNNN920320.20620456800745715No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGANNNNNNN901500.20198780647896672No Hit
GACTTGGGCCAACGTCTATAGANNNNNNNNNNNNN897550.20110277948441105No Hit
TGAGTCTGAATATTCGTGCATGGAATAGANNNNNN823940.1846099093402993No Hit
GTGCGAAAGGGAAGTGGGTTAGANNNNNNNNNNNN819020.18350754660277682No Hit
AATATTCCCGCACTTTGTGAGANNNNNNNNNNNNN801460.17957309748267627No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGAGANNNNNNN771940.17295892105754138No Hit
TCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNN734270.16451867627655117No Hit
GAGCAGAGCGCTATGTGGCATAGANNNNNNNNNNN718710.16103234209040282No Hit
AATGAATCCAGAGACGTTGGCACAGANNNNNNNNN718430.16096960599964952No Hit
ATCTGACGAGCGCGGCGAGCAGANNNNNNNNNNNN717180.16068953416592938No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAN712630.15967007269118808No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGANN684670.15340542591453593No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTAGANNNN651030.14586813272545945No Hit
AGTGATGGCCGCATCGGCGACTCAGGAGANNNNNN630690.1413108038471653No Hit
TCTCCTTCGGGGGAGTGTTATAGANNNNNNNNNNN619610.13882824711306996No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGANNN607590.13613507636001707No Hit
AAATGAATCCAGAGACGTTGGCAGANNNNNNNNNN596460.13364131675257293No Hit
ACGTGAAACCGTTGAGGTGCAGANNNNNNNNNNNN596330.13361218928186602No Hit
TTGGGAATACCTCGTGCGCTAGANNNNNNNNNNNN593740.1330318804423979No Hit
AAATGAATCCAGAGACGTTGGCACAAGANNNNNNN581600.13031182279330786No Hit
TGGAGCAGAGCGCTATGTGGCATAGANNNNNNNNN580200.12999814233954132No Hit
GAGCGCTATGTGGCATATAGCTCGAGAGANNNNNN555730.1245154561226358No Hit
TGAGTCTGAATATTCGTGCAGANNNNNNNNNNNNN550420.12332571097299264No Hit
CTCCTTCGGGGGAGTGTTATAGANNNNNNNNNNNN549340.12308372890865843No Hit
CTTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNN537850.12050930861310287No Hit
GTGACATCCGGCGCGCTTGTGGCGAGANNNNNNNN515180.11542992583675436No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTTAGANNNNN511430.11458971033559394No Hit
GGAGCAGAGCGCTATGTGGCATAGANNNNNNNNNN510810.11445079470606875No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTAGANNNNNN480540.10766857518070179No Hit
GACTTGGGCCAACGTCTATCTGAGAGANNNNNNNN479330.10739746564566069No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGAN479170.1073616164509445No Hit
TGACCTCAGATTAGTCGAGAGCAGANNNNNNNNNN476580.10678130761147638No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGANN461030.1032972139999978No Hit
TTGAATCAACTGTAGACGACTTTAAGCAGANNNNN451620.10118883323575256No Hit
AAAGCGAGAGCTTAGAGTCGAGAGANNNNNNNNNN447960.10036878290661999No Hit
AGGTGGGAGCTCCGGCGACAATGAAATAGANNNNN447330.10022762670242505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)