FastQCFastQC Report
Fri 7 Jul 2023
SRR7252577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7252577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108285892
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3341584030.85890450068971No Hit
AGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNNN22780112.103700637198427No Hit
TAGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNN19240901.776861199979772No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATAGANNNNN7166340.6617981223260367No Hit
ATCCGGCGCGCTTGTGGCGAGANNNNNNNNNNNNN7046990.6507763726044755No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGANNNNNN4943610.4565331557688051No Hit
TTTTTGGAGCAGAGCGCTATGTGGCAGANNNNNNN4757040.4393037645199432No Hit
ATTGCGATCCGTTGAAACTCAGANNNNNNNNNNNN3824130.35315126738762975No Hit
TCCTTCGGGGGAGTGTTATAGANNNNNNNNNNNNN3791670.35015364697739204No Hit
AACGTCTATCTGAGGGTCACTAGANNNNNNNNNNN3285290.3033903991851496No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNN3078530.2842964991228959No Hit
AGAGCGCTATGTGGCATATAGANNNNNNNNNNNNN2713190.2505580320657099No Hit
AAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNNN2697320.24909246718861586No Hit
AAATGAATCCAGAGACGTTGGCACAGANNNNNNNN2635140.24335026025366258No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGANNNNN2484180.22940938603525562No Hit
TGGGAATACCTCGTGCGCTAGANNNNNNNNNNNNN2466260.22775450748468692No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATATAGANNN2446320.22591308570464563No Hit
TGAGTCTGAATATTCGTGCATGGAATAAGANNNNN2414790.223001349058472No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGN2385520.22029831919378748No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTGAGANNN2372320.2190793238328775No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGANNNNN2313830.2136778815101786No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGANNNNNNN2193730.2025868706885658No Hit
TTGTGACATCCGGCGCGCTTGTGGCAGANNNNNNN2148470.19840719417077896No Hit
GACTTGGGCCAACGTCTATAGANNNNNNNNNNNNN2125320.19626933488251638No Hit
GTGCGAAAGGGAAGTGGGTTAGANNNNNNNNNNNN1983610.18318268089808043No Hit
TGAGTCTGAATATTCGTGCATGGAATAGANNNNNN1982540.18308386839534No Hit
AATATTCCCGCACTTTGTGAGANNNNNNNNNNNNN1954010.1804491761493732No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGAGANNNNNNN1861010.1718607997429619No Hit
TCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNN1798590.1660964292559921No Hit
GAGCAGAGCGCTATGTGGCATAGANNNNNNNNNNN1774520.16387360968499942No Hit
ATCTGACGAGCGCGGCGAGCAGANNNNNNNNNNNN1737780.16048073926379994No Hit
AATGAATCCAGAGACGTTGGCACAGANNNNNNNNN1733200.16005778481281752No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAN1728300.1596052789591464No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGANN1687320.1558208524523213No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTAGANNNN1582310.14612337496374875No Hit
AGTGATGGCCGCATCGGCGACTCAGGAGANNNNNN1531500.1414311663055793No Hit
TCTCCTTCGGGGGAGTGTTATAGANNNNNNNNNNN1531480.14142931934291125No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGANNN1502290.13873367732889896No Hit
TGGAGCAGAGCGCTATGTGGCATAGANNNNNNNNN1436080.13261930741633451No Hit
ACGTGAAACCGTTGAGGTGCAGANNNNNNNNNNNN1427150.13179463858505225No Hit
AAATGAATCCAGAGACGTTGGCAGANNNNNNNNNN1421310.13125532548598298No Hit
TTGGGAATACCTCGTGCGCTAGANNNNNNNNNNNN1417050.1308619224376893No Hit
AAATGAATCCAGAGACGTTGGCACAAGANNNNNNN1390070.128370369798496No Hit
GAGCGCTATGTGGCATATAGCTCGAGAGANNNNNN1367280.12626575583825822No Hit
CTCCTTCGGGGGAGTGTTATAGANNNNNNNNNNNN1342390.12396721079787568No Hit
TGAGTCTGAATATTCGTGCAGANNNNNNNNNNNNN1337080.12347684220950961No Hit
CTTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNN1322500.1221304064245045No Hit
GTGACATCCGGCGCGCTTGTGGCGAGANNNNNNNN1269470.11723318491018203No Hit
GGAGCAGAGCGCTATGTGGCATAGANNNNNNNNNN1227150.11332501190459787No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTTAGANNNNN1223570.11299440558701776No Hit
TGACCTCAGATTAGTCGAGAGCAGANNNNNNNNNN1164590.10754771267895175No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTAGANNNNNN1154090.1065780572782279No Hit
GACTTGGGCCAACGTCTATCTGAGAGANNNNNNNN1140750.1053461331786416No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGAN1138810.10516697779984119No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGANN1130520.10440141177393635No Hit
TTGAATCAACTGTAGACGACTTTAAGCAGANNNNN1115200.10298663837021356No Hit
CAAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNN1096380.10124864649958278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)