FastQCFastQC Report
Thu 28 Sept 2023
SRR7252578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7252578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42593441
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38163628.959975785943193No Hit
AAATGAATCCAGAGACGTTGGCACAGANNNNNNNN2259490.5304783898534988No Hit
AATGAATCCAGAGACGTTGGCACAGANNNNNNNNN2208110.5184154997009985No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGANN2092770.4913362130098857No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNN1913330.44920766086966296No Hit
ATTGCGATCCGTTGAAACTCAGANNNNNNNNNNNN1344360.31562606082941264No Hit
ATGAATCCAGAGACGTTGGCACAGANNNNNNNNNN1219570.28632812267973373No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGANNNNNN1126340.2644397760678692No Hit
ATACCGCGTAGAATAAACCAGTTCTAGANNNNNNN1085100.25475753414710023No Hit
AATCCAGAGACGTTGGCACAGANNNNNNNNNNNNN1019650.23939131848962378No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGANNN1012270.23765865735055314No Hit
TGAATCCAGAGACGTTGGCACAGANNNNNNNNNNN995020.23360873802142448No Hit
GTGTAACGGTTAGCACTCTGAGANNNNNNNNNNNN984390.23111304860295276No Hit
TCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNN976780.2293263885394937No Hit
CAAATGAATCCAGAGACGTTGGCACAGANNNNNNN963260.22615219089718533No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCAGANNNN924170.21697472153048167No Hit
GTGTAATGGCTATCACTGTGAGANNNNNNNNNNNN918000.21552614168928028No Hit
TAGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNN901720.21170395695431135No Hit
ATCTGACGAGCGCGGCGAGCAGANNNNNNNNNNNN809760.19011377831624357No Hit
AAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNNN799930.18780591124347057No Hit
AGAGACGTTGGCACAAGTTTCGGAGANNNNNNNNN790590.185613085357438No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGANNN786690.18469745142215677No Hit
AGAGACGTTGGCACAAGTTTCGAGANNNNNNNNNN778270.18272062123367774No Hit
GAATCCAGAGACGTTGGCACAGANNNNNNNNNNNN750750.1762595325416418No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGANNNN728400.17101224575868385No Hit
ATGGGTGACCGGTTGGGAATACCTCGTGCGCAGAN693800.16288892930721421No Hit
GACTTGGGCCAACGTCTATCTGAGAGANNNNNNNN648130.15216662114713858No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGAGANNNNNNN641640.15064291236765773No Hit
ATACCGCGTAGAATAAACCAGANNNNNNNNNNNNN641260.15055369675345084No Hit
AATGAATCCAGAGACGTTGGCAGANNNNNNNNNNN639300.15009353200648898No Hit
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGAN636190.1493633726375852No Hit
AAATGAATCCAGAGACGTTGGCAGANNNNNNNNNN626540.1470977655925944No Hit
ATCTGACGAGCGCGGCGAGCCAGANNNNNNNNNNN623490.14638169289961805No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTGAGANNN617960.14508337093497567No Hit
ATGGGTGACCGGTTGGGAATACCTCGTGCGCTAGN616020.14462790174665624No Hit
TTGTGACATCCGGCGCGCTTGTGGCAGANNNNNNN614680.14431329931761092No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGN594350.13954026395754218No Hit
AATGAATCCAGAGACGTTGGCACAAGAGANNNNNN593510.13934305049455853No Hit
AAATGAATCCAGAGACGTTGGCACAAGANNNNNNN592690.13915053259021734No Hit
AATATTCCCGCACTTTGTGAGANNNNNNNNNNNNN576710.13539878123488544No Hit
ATCCGGCGCGCTTGTGGCGAGANNNNNNNNNNNNN566240.13294065628555346No Hit
CGTCCGATCACTGAAGTCAAACTACAGANNNNNNN566150.13291952627166234No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGANNNNNNN565580.13278570285035202No Hit
AAATGAATCCAGAGACGTTGGCACAAGAGANNNNN564430.1325157082284101No Hit
AATGAATCCAGAGACGTTGGCACAAGTTTCAGANN542030.12725668254884595No Hit
ATCTAAAGCTAAATACTGATATGAGACCAGANNNN536810.12603114174316182No Hit
ATTTCCAAGGCCGAAAGGTCGAGGTAATCCGTTGAACCC520040.12209391582145243No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGANNN509780.11968509423786633No Hit
CGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNNN509360.11958648750637453No Hit
CAAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNN499630.11730209822681384No Hit
TGCGAGAGGTCCCGGGTTCAGANNNNNNNNNNNNN497780.11686775905238557No Hit
ATGAATCCAGAGACGTTGGCACAAGTTTCAGANNN493980.11597560291031664No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGANNNNNNN487680.11449650193793921No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTTAGANNNNN479910.11267227740534042No Hit
AGAGACGTTGGCACAAGTTTCAGANNNNNNNNNNN479090.11247975950099923No Hit
GACTTGGGCCAACGTCTATCTGAAGANNNNNNNNN474750.11146082327558368No Hit
AAATGAATCCAGAGACGTTGGCACAAGTTTCAGAN472990.1110476141150465No Hit
AATGAATCCAGAGACGTTGGCACAAGANNNNNNNN471790.1107658805964984No Hit
AGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNNN467990.1098737244544295No Hit
GTCATACCGCGTAGAATAAACCAGTTCTAGANNNN456920.10727473274582347No Hit
ATGAATCCAGAGACGTTGGCAGANNNNNNNNNNNN447220.1049973868042265No Hit
TTGCGATCCGTTGAAACTCAGANNNNNNNNNNNNN437360.10268247639348979No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTAGANNNN434280.10195936036254971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)