FastQCFastQC Report
Thu 28 Sept 2023
SRR7252579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7252579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95442379
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83562998.755333938186935No Hit
AAATGAATCCAGAGACGTTGGCACAGANNNNNNNN4971140.5208524821033642No Hit
AATGAATCCAGAGACGTTGGCACAGANNNNNNNNN4889800.5123300625186638No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGANN4647710.48696502001485104No Hit
TTCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNN4225110.4426869954698007No Hit
ATTGCGATCCGTTGAAACTCAGANNNNNNNNNNNN2966650.31083152275573517No Hit
ATGAATCCAGAGACGTTGGCACAGANNNNNNNNNN2717800.2847581994996164No Hit
ATACCGCGTAGAATAAACCAGTTCTAGANNNNNNN2424000.2539752283416992No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGANNNNNN2413190.25284260778956485No Hit
AATCCAGAGACGTTGGCACAGANNNNNNNNNNNNN2284830.23939365551648709No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGANNN2260330.23682666166567368No Hit
TGAATCCAGAGACGTTGGCACAGANNNNNNNNNNN2220310.23263355579181444No Hit
GTGTAACGGTTAGCACTCTGAGANNNNNNNNNNNN2193230.22979624177222155No Hit
TCGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNN2189020.22935513793091852No Hit
CAAATGAATCCAGAGACGTTGGCACAGANNNNNNN2167400.22708989682664973No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCAGANNNN2103900.22043666786637833No Hit
GTGTAATGGCTATCACTGTGAGANNNNNNNNNNNN2045450.2143125539651521No Hit
TAGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNN1997860.20932629937902114No Hit
ATCTGACGAGCGCGGCGAGCAGANNNNNNNNNNNN1797590.18834295821565805No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGANNN1781660.18667388833633328No Hit
AAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNNN1757170.18410794223811205No Hit
AGAGACGTTGGCACAAGTTTCGGAGANNNNNNNNN1745900.18292712506673792No Hit
AGAGACGTTGGCACAAGTTTCGAGANNNNNNNNNN1719920.18020506383228357No Hit
GAATCCAGAGACGTTGGCACAGANNNNNNNNNNNN1662260.1741637223858387No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGANNNN1651660.17305310463813983No Hit
ATGGGTGACCGGTTGGGAATACCTCGTGCGCAGAN1525350.15981894164645666No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGAGANNNNNNN1424360.1492376882181447No Hit
ATACCGCGTAGAATAAACCAGANNNNNNNNNNNNN1419300.1487075254065073No Hit
GACTTGGGCCAACGTCTATCTGAGAGANNNNNNNN1411840.14792590197274946No Hit
AATGAATCCAGAGACGTTGGCAGANNNNNNNNNNN1407090.1474282194914693No Hit
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGAN1397300.1464024697037361No Hit
AAATGAATCCAGAGACGTTGGCAGANNNNNNNNNN1378120.14439288023195648No Hit
AAGTTCTGTGTCGCGGAAGAGCGTGCGTGAGANNN1373510.14390986628696673No Hit
ATCTGACGAGCGCGGCGAGCCAGANNNNNNNNNNN1359580.14245034692607567No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGN1347740.14120980785694792No Hit
ATGGGTGACCGGTTGGGAATACCTCGTGCGCTAGN1341970.14060525461126655No Hit
TTGTGACATCCGGCGCGCTTGTGGCAGANNNNNNN1323910.13871301342980982No Hit
AATGAATCCAGAGACGTTGGCACAAGAGANNNNNN1311270.13738865415330856No Hit
AAATGAATCCAGAGACGTTGGCACAAGANNNNNNN1298580.13605905611384644No Hit
AATATTCCCGCACTTTGTGAGANNNNNNNNNNNNN1290200.13518103944160906No Hit
CGTCCGATCACTGAAGTCAAACTACAGANNNNNNN1278360.13394050037248129No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGANNNNNNN1261080.13212998389321373No Hit
ATCCGGCGCGCTTGTGGCGAGANNNNNNNNNNNNN1257930.1317999418266806No Hit
AAATGAATCCAGAGACGTTGGCACAAGAGANNNNN1246790.1306327454390046No Hit
ATCTAAAGCTAAATACTGATATGAGACCAGANNNN1212810.1270724821308153No Hit
AATGAATCCAGAGACGTTGGCACAAGTTTCAGANN1200990.12583403856687184No Hit
ATTTCCAAGGCCGAAAGGTCGAGGTAATCCGTTGAACCC1161720.12171951413742527No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGANNN1133560.11876904283787813No Hit
CGGTGCGAAAGGGAAGTGGGTTAGANNNNNNNNNN1119000.11724351506368046No Hit
TGCGAGAGGTCCCGGGTTCAGANNNNNNNNNNNNN1114340.11675526235572983No Hit
CAAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNN1111900.11649961072324067No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGANNNNNNN1096800.1149175043090659No Hit
ATGAATCCAGAGACGTTGGCACAAGTTTCAGANNN1094480.11467442570768274No Hit
AGAGACGTTGGCACAAGTTTCAGANNNNNNNNNNN1059920.11105339274914763No Hit
AAATGAATCCAGAGACGTTGGCACAAGTTTCAGAN1058100.11086270177737291No Hit
AAAGCGAGAGCTTAGAGTCGAGTTGTTAGANNNNN1053930.11042578894643856No Hit
AATGAATCCAGAGACGTTGGCACAAGANNNNNNNN1040670.10903646900922283No Hit
GTCATACCGCGTAGAATAAACCAGTTCTAGANNNN1039260.10888873589372705No Hit
GACTTGGGCCAACGTCTATCTGAAGANNNNNNNNN1038600.10881958422264391No Hit
ATGAATCCAGAGACGTTGGCAGANNNNNNNNNNNN1002750.10506339117971902No Hit
AGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNNN1002000.10498480973530637No Hit
TTGCGATCCGTTGAAACTCAGANNNNNNNNNNNNN987850.10350223981738763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)