FastQCFastQC Report
Thu 28 Sept 2023
SRR7252580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7252580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40180747
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN930336223.153780590490268No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGANNN1369560.3408498104826174No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCAGANNNN1144420.28481800002374275No Hit
AGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNNN823850.20503600891242763No Hit
TAGGTGGGAGCTCCGGCGACAGANNNNNNNNNNNN800780.1992944531369713No Hit
GTGTAATGGCTATCACTGTGAGANNNNNNNNNNNN794980.19785097574218816No Hit
GTGTAACGGTTAGCACTCTGAGANNNNNNNNNNNN715380.17804049287585422No Hit
ATACCGCGTAGAATAAACCAGANNNNNNNNNNNNN711820.17715449640595282No Hit
AATATTCCCGCACTTTGTGAGANNNNNNNNNNNNN689050.1714876032543646No Hit
ATGGGTGACCGGTTGGGAATACCTCGTGCGCAGAN672640.1674035577287799No Hit
AATGAATCCAGAGACGTTGGCACAGANNNNNNNNN668570.16639063479830277No Hit
AAAGCGAGAGCTTAGAGTCGAGTTAGANNNNNNNN593900.14780710771753447No Hit
GCCTGCCACGCGGGAGGCCCGGGTTCAGANNNNNN588090.1463611415686224No Hit
GCCTGCCACGCGGGAGGCCCGGGTTCGATTCCCGGACGTTGC583800.14529346604730867No Hit
AAAGCGAGAGCTTAGAGTCGAGAGANNNNNNNNNN583120.145124230766541No Hit
AAATGAATCCAGAGACGTTGGCACAGANNNNNNNN568520.1414906497382938No Hit
ATACCGCGTAGAATAAACCAGTTCTAGANNNNNNN562760.14005712736002643No Hit
ATCTGACGAGCGCGGCGAGCAGANNNNNNNNNNNN545550.13577398150412684No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGANN526810.1311100562665No Hit
ATCTGACGAGCGCGGCGAGCCAGANNNNNNNNNNN506280.1260006440397935No Hit
GGGAGAGCGACGCTCAGACAGANNNNNNNNNNNNN496480.12356166499343578No Hit
TGCGAGAGGTCCCGGGTTCAGANNNNNNNNNNNNN477200.11876334703284634No Hit
GTGCGAAAGGGAAGTGGGTTAGANNNNNNNNNNNN471830.11742688606560749No Hit
ATGAATCCAGAGACGTTGGCACAGANNNNNNNNNN443560.1103911781431042No Hit
GGGAGAGCGACGCTCAGACAGGAGANNNNNNNNNN431610.10741711695902519No Hit
AATCCAGAGACGTTGGCACAGANNNNNNNNNNNNN425590.10591888697340544No Hit
ATGGGTGACCGGTTGGGAATACCTCGTGCGCTAGN419920.10450776338229849No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGANN417860.10399508003173759No Hit
TGAATCCAGAGACGTTGGCACAGANNNNNNNNNNN411890.10250929381676255No Hit
GCCTGTCACGCGGGCGACCGGGGTTCAGANNNNNN408800.1017402687909212No Hit
ACGTGAAACCGTTGAGGTGCAGANNNNNNNNNNNN403900.10052077926774235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)