FastQCFastQC Report
Tue 4 Jul 2023
SRR7262143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7262143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58890769
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG678735911.525335999602927No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG38181496.483442252214434No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG18677713.1715853464233077Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA14246112.419073522371562No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG13797992.3429801027050603Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA13482352.289382568599164No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA11047601.8759476548862861No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA8872691.5066351060893772No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGGA6128711.0406911140861481RNA PCR Primer, Index 1 (100% over 22bp)
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA5145800.8737871974468527RNA PCR Primer, Index 1 (100% over 22bp)
ATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGAA4680080.7947051939498361RNA PCR Primer, Index 1 (100% over 23bp)
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG4206100.714220593723271No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA4100170.6962330547933582No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTGGAATTCTCGGGTGCCAA2936230.4985891761746225No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA2822320.4792465861670103RNA PCR Primer, Index 1 (100% over 22bp)
ACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAGGA2432330.41302398343618163RNA PCR Primer, Index 1 (100% over 22bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG1766760.3000062709318671Illumina Small RNA Adapter 2 (100% over 21bp)
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA1533220.26034980117172524No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC1506540.2558193797741035No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC1469970.24960957803081157RNA PCR Primer, Index 8 (100% over 50bp)
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTG1299720.22070012364756184No Hit
TGCTGGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG1118860.1899890286710299No Hit
TCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCAAGG1082010.18373168127588893Illumina Small RNA Adapter 2 (100% over 21bp)
TTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAG1024980.17404765082962323No Hit
AGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGAA1007190.1710268038782105RNA PCR Primer, Index 1 (100% over 23bp)
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC931660.15820136429191475No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGTGCCAAGG863420.1466138097126903No Hit
TACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCAAGG828320.1406536226416062Illumina Small RNA Adapter 2 (100% over 21bp)
TATGTATGTAAGCGTATTACCGGTGGAGTGGAATTCTCGGGTGCCAAGGA822480.13966195618875346RNA PCR Primer, Index 1 (100% over 22bp)
ACCTCGGCTGAGTTGACCTCTGCGATTATTCCTGGAATTCTCGGGTGCCA797670.13544907182312393No Hit
CTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA778350.13216842184553576No Hit
TCGGCTGAGTTGACCTCTGCGATTATTCCTTGGAATTCTCGGGTGCCAAG718700.12203949994268203No Hit
TAAGGGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG715520.12149951718239575No Hit
TGGTTTATGTGCTCGTTCTGGGTAAATAGAGTGGAATTCTCGGGTGCCAA696590.11828509150559742No Hit
TGCTCGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG662380.1124760316850337No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGG646950.10985592665634913Illumina Small RNA Adapter 2 (100% over 21bp)
TAGTTACGTAGCCAATTGTGGAACTTTCTTGCTGGAATTCTCGGGTGCCA644530.10944499637965333No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTGGAATTCTCGGGTGCCAAGGA631120.10716789926788017RNA PCR Primer, Index 1 (100% over 22bp)
TGCTAGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG589320.1000700126704068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)