FastQCFastQC Report
Sun 2 Jul 2023
SRR7262146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7262146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64557522
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG58192499.01405261496871No Hit
TTCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAAG8683251.345040783938392No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTG5484960.8496236890876946No Hit
TAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGG4924930.7628746964606231Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGG4903460.759548980210238Illumina Small RNA Adapter 2 (100% over 21bp)
TAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAG4595470.7118411391317033No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAA3754980.5816487194164608No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGTGC3645410.5646762587944438No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCA3599430.5575539284175127No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAA3573780.5535807275874064No Hit
TTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAAGGA3450680.5345124616152398RNA PCR Primer, Index 1 (100% over 22bp)
TGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCAAG3326130.5152195897481938No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAAGG2968580.45983487408330204Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCTGGAATTCTCGGGTG2744020.42505039149427076No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAG2497110.38680388011175526No Hit
CTGCTTGGACTACATATGGTTGAGGGTTGTATGGAATTCTCGGGTGCCAA2108930.3266745585433096No Hit
GTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCAA2021970.31320440087523804No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCCA1884670.2919365461394258No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCTGGAATTCTCGGGTGCCAAGGA1676390.2596738455977291RNA PCR Primer, Index 1 (100% over 22bp)
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGTGGAATTCTCGGGTGC1652770.2560150930204539No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAGG1295700.20070472965179795Illumina Small RNA Adapter 2 (100% over 21bp)
ATATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAA1129920.17502530533932203No Hit
AATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGTGGAATTCTCGGGT1063840.16478947255751233No Hit
TGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGGAATTCTCGGGTGCCA954490.14785109007127006No Hit
ACATTTTGAACGCATATCGCAGTCCATGCTGTGGAATTCTCGGGTGCCAA946750.1466521592944661No Hit
TATGGCAAATGTGAGATGCAGTGTATGGAGTGGAATTCTCGGGTGCCAAG926430.14350457875381276No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC872270.13511516132852808RNA PCR Primer, Index 7 (100% over 50bp)
TCGACATTTTGAACGCATATCGCAGTCCATGCTGGAATTCTCGGGTGCCA803550.1244703909174209No Hit
TATGGCAAATGTGAGATGCAGTGTATGGAGCGTGGAATTCTCGGGTGCCA787650.12200747110460651No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCATGGAATTCTCGGGT779890.1208054423154594No Hit
ACGGCAATTTTTGGAAGCCCGAGAGGGCTAATGGAATTCTCGGGTGCCAA746960.11570456499244194No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGGAATTCTCGGGTGCC706400.1094217959605079No Hit
TGGAACGTACCTTGAGCATATATGCTGTGTGGAATTCTCGGGTGCCAAGG664370.10291132302135142Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTTGGAATTCTCGGGTGCCA662290.10258912973766247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)