FastQCFastQC Report
Fri 7 Jul 2023
SRR7277902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7277902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92082823
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT16741121.8180502567780747No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA14153591.5370499664199044No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG13675201.4850978232932759No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA11887501.2909573808352943No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT11736711.2745819054656915No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG11418391.240013026099341No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA10868551.180301563951835No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA9214491.0006741431026718No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT8624070.9365557787037003No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA8384960.9105889379607747No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG8099840.8796255084403743No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA7969950.8655197289075293No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA7746870.8412937122920308No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT7276780.7902429316268899No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA6468810.7024990969271218No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG5656350.6142676577150551No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA5431940.5898972059099448No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC5301760.575759932989891No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA5150840.5593703398949877No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT4945950.5371197188426771No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG4839160.5255225504978274No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC4557020.49488274268046706No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT4331320.47037219960122206No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGA4142020.4498146196060909No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC4068920.4418761140717852No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT3636760.39494445125775524No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA3473980.3772668872239071No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA3445130.37413383818608603No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3401430.3693881105274108No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3335600.3622391116310585No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA3223410.35005551469680724No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA3050960.33132780909638276No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA3014010.3273151171744594No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC2753520.2990264536090515No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA2634020.2860490061213697No Hit
TGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA2623120.284865289153874No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA2618430.2843559650641901No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2585130.2807396554295474No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGAT2571320.2792399186110964No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC2511550.272749022909517No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC2389430.25948704895808855No Hit
TTTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2372160.25761156345087294No Hit
TTGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA2371360.2575246851413319No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC2351440.25536141523376193No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA2268110.24631195331620098No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA2243220.2436089519106077No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATC2203150.23925743458147455No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCAT2107610.22888199246454466No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG2101960.22826841440341158No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT2093350.2273333865969769No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCAT2028550.2202962435241587No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA1990360.2161488902224468No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1983110.21536155554223183No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC1977150.2147143121361516No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1962550.21312878298702898No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA1880120.20417705916770168No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1842590.2001013804713611No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT1808390.1963873327384848No Hit
CCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1793350.1947540205191146No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC1781630.1934812532843395No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCA1778890.19318369507416167No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC1778460.1931369979827834No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG1738710.1888202319774666No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA1699200.18452952946501217No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG1696230.18420699374084132No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAAT1682420.1827072569223904No Hit
TCTAGACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC1677850.18221096457913763No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGAT1675520.18195793150259956No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1656810.1799260650382102No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATC1630460.17706451071770465No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1605270.17432892994603347No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGA1519410.16500471537454928No Hit
GCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCACGTCTGTAGGCACCAT1486320.16141121129616107No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTCTGTAGGCACC1484700.1612352827193406No Hit
CCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1459360.1584834122646305No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAATA1453900.1578904678020134No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT1451360.15761462916922084No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC1439760.15635489368087682No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATC1434110.15574131561974378No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA1371820.14897675324311027No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1371300.14892028234190866No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT1356760.1473412690660016No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1353860.14702633519391559No Hit
TGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCACGTCTGTAGGCACCA1335720.14505636952507417No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATA1306740.14190920276195268No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1281950.13921706114505197No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC1276730.13865018017529718No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT1269580.1378737052837748No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGAT1259420.13677035075260455No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1203500.130697556915691No Hit
TCGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATC1198600.13016542726975258No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG1169440.12699871288698436No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCCTGTAGGCACCATCAATAGAT1147900.12465951440259385No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCA1144030.12423924058018943No Hit
GGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAG1141390.12395254215870424No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTGTAGGCACCATCAA1136160.12338457521008017No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT1122380.12188809632823702No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCAT1115420.12113225503523062No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATC1115240.1211127074155839No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACC1102170.11969333303345837No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT1094570.11886798909281919No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1089370.11830328008080292No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1062940.11543303792934324No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA1057770.11487158685393474No Hit
GGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGA1055300.11460335007322701No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCA1047930.11380298364658085No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGAT1042120.11317202992353959No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAG989910.10750213424712228No Hit
TTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGATC986770.10716113688217399No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG984590.10692439348867486No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA972090.10556691990209727No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA940480.10213414069635984No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCG935510.1015944091983366No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT933660.1013935031075231No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT929500.10094173589791008No Hit
TACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCCTGTAGGCACCATCA928260.10080707451812158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)