FastQCFastQC Report
Fri 7 Jul 2023
SRR7277903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7277903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94386401
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT18558391.9662143914142887No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA16589821.7576493884961246No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA15408661.63250847969084No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT14639231.5509893210145813No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA10853471.149897642563996No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA10631041.1263317477270904No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG10442181.1063225093199602No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG7959920.843333352651088No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT7194240.7622114969718996No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT6924670.7336512385931528No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA6815040.7220362179081286No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT5424830.5747469913594861No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA5103400.5406922973999189No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA5060050.5360994747537836No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG4926260.5219247632929663No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC4836030.5123651234461202No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT4833100.5120546973710757No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC4412120.4674529331826096No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC4204900.4454984993018221No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG4155970.440314489796046No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC4043470.4283953998839303No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA3995640.4233279325906282No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT3747720.3970614368483019No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3741770.3964310494262833No Hit
TTGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA3718490.39396459242046955No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGA3524930.3734574009236775No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA3477610.36844396683797703No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA3436980.36413932129905024No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3379580.3580579367572242No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA3285430.34808298284410694No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA3175120.3363959178822805No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA3170080.3358619426542177No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCAT3160270.33482259801388126No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3049610.3230984514389949No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA3037900.321857806613476No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2971710.31484514384651663No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT2917060.30905511483587556No Hit
TTTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2888130.3059900546478088No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC2883860.30553765896847784No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2865770.30362106931060967No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG2804740.29715509546761937No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGAT2639340.27963138461016224No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2608660.27638091635679596No Hit
TGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA2602680.27574735051080085No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA2576620.2729863595498254No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATC2549860.27015120536273013No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT2498360.2646949108696283No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2475710.2622952007673224No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA2390150.2532303355861614No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATC2382760.25244738381326776No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2381980.2523647447898771No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA2370770.25117707369730097No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC2358210.2498463735257794No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC2339000.2478111227061195No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA2314900.2452577887782796No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2245850.23794211625888778No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC2240870.2374144978787781No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCAT2223180.2355402872072641No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCAT2184080.23139774129114213No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA2121260.2247421214842168No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG2073420.2196735947162558No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2012050.21317159873486435No Hit
CCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1829090.19378745037645836No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCA1825880.19344735901096602No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACC1807050.19145236822834255No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT1784760.18909079921375538No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT1776310.18819554312702313No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1769900.18751641987069725Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1765720.18707355946329599No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT1758190.1862757750451784No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAAT1728200.18309841054327308No Hit
TGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC1715430.1817454614039156No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA1710010.18117122613881634No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1700930.18020922314857624No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAATA1673990.17735499841762162No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1650120.1748260324069354No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG1619940.17162853788651183No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA1606980.17025545872863612No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC1572640.16661722275012902No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGA1558750.1651456124489798No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC1524410.1615073764704727No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCA1487920.15764135344031183No Hit
GCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCACGTCTGTAGGCACCAT1426910.15117749854663914No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1423500.15081621768796968No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1381500.14636642412077985No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1381080.14632192618510798No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTGTAGGCACCATCAA1371660.1453239010564668No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA1357970.1438734802484947No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1352980.14334480239372618No Hit
TGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCACGTCTGTAGGCACCA1348470.14286697932258272No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA1346130.1426190622524107No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC1334100.14134451423780847No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1291710.1368534011589233No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1282260.1358521976063056No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1282120.13583736496108162No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG1268880.13443462051275798No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTCTGTAGGCACC1244560.13185797814242328No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCA1243890.13178699334028002No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATA1242820.13167362955178258No Hit
TTTAATGCTAATCGTGATAGGGGTCTGTAGGCACCATCAATAGATCGGAA1211390.12834370069900217No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGA1205940.12776628700992637No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1200090.12714649433449635No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT1187510.1258136752136571No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGAT1174240.12440775234135688No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT1168010.1237476996288904No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATC1147000.12152174337063662No Hit
TCGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATC1146580.12147724543496473No Hit
TCTAGACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC1107990.11738873272644436No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACC1105330.117106912467189No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATC1101630.11671490684341274No Hit
TTGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT1045590.11077761085519088No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGCTGTAGGCACCATCAAT1041190.1103114420052948No Hit
CTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT959540.101660831415746No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCCTGTAGGCACCATCAATAGAT948480.10048905244305269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)