FastQCFastQC Report
Fri 7 Jul 2023
SRR7277904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7277904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73900870
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT19950992.699696228204079No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA12436081.6828056286752782No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA10978371.485553553023124No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA9900231.339663524935498No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT9051431.2248069610005943No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA9011541.2194091896347092No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG8507261.1511718332950613No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA8072291.0923132569345935No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC7754071.0492528707713455No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT7251960.9813091510289392No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA7192180.9732199363823458No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG6902390.9340065955921764No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA6185170.836954964129651No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT5999080.8117739344611234No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG5642880.7635742312641245No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT5423550.7338952843180331No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC4740760.6415025966541396No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGA4129230.5587525559577309No Hit
TTTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA3892410.5267069251011525No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA3809890.5155406154217129No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT3771980.5104107705362603No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGAT3683760.4984731573525454No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC3621520.4900510643514751No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC3365840.45545336611057485No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3294750.44583372293181395No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT3274780.4431314543387649No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT3264810.4417823497883043No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC3100760.41958369366964154No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG2888830.3909060881150655No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2844370.38488992078171747No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA2750270.3721566471409606No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC2711750.36694425924891005No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG2670040.36130021202727386No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC2520720.3410947665433438No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA2296960.31081636792638573No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA2188910.296195430446218No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGA2144420.29017520362074223No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA2100180.2841888058963311No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC2091210.28297501775012934No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT2074060.2806543414170902No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC2031670.274918279040558No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2024560.27395617940627764No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1952360.26418633501878935No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1916100.2592797622003638No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCAT1914210.2590240141963146No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA1912480.2587899168169468No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1775690.24027998587838004No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCA1693750.22919215971341067No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC1579880.21378368076045653No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1563290.21153878161380238No Hit
TGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA1528970.206894722619639No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG1513330.20477837405703075No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA1507780.2040273680133942No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGAT1502450.20330613157869454No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1494980.20229531803888098No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1487430.20127367918672678No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGA1470300.19895570918177283No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTCTGTAGGCACC1468570.19872161180240502No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1467260.19854434731282597No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1462120.19784882099493553No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG1453250.1966485644891596No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCAT1451010.19634545574361978No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATC1428220.19326159489056083No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTGTAGGCACCATCAA1389460.18801673106148817No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1370640.18547007633333681No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA1357170.18364736436797024No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG1346520.18220624466261356No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1326600.17951074188977748No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC1309220.17715894278375882No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA1296320.1754133611688198No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCA1293370.17501417777625622No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1290330.17460281590730933No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA1285790.1739884794319742No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA1222230.16538776877728234No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGCTGTAGGCACCATCAAT1190320.16106982231738273No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1179210.159566457066067No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA1168100.1580630918147513No Hit
TCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGCTGTAGGCA1166590.15785876404432045No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATC1130570.15298466716291703No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATC1096050.14831354488790185No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1080250.1461755457006122No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAAT1074930.14545566242995517No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT1069800.1447614892761073No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT998500.135113429652452No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCAT997570.13498758539649125No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAG994470.13456810454328885No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATC986930.1335478188551772No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCA978760.1324422838323825No Hit
TTTAATGCTAATCGTGATAGGGGTCTGTAGGCACCATCAATAGATCGGAA977780.1323096737562088No Hit
CCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG973040.13166827400002193No Hit
TTGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA966330.13076030092744512No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA953910.1290796711865503No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGAT953310.128998481343995No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA946590.1280891551073756No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA946280.12804720702205535No Hit
TCTAGACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC928650.125661578814972No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGATC927550.12551273077028727No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC879790.11905001930288507No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT868370.11750470596624911No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACC858190.11612718497089412No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACC835070.11299866970442972No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG833840.11283223052719137No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCG828990.11217594596653598No Hit
TTGACCGGGGTCCGGTGCGGAGAGCCGTTCGCTGTAGGCACCATCAATAG827160.1119283169467423No Hit
CCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA825380.11168745374716157No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC823260.11140058297013282No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATC813020.11001494299052231No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA808090.10934783311752623No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA807900.10932212300071703No Hit
CTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT803710.1087551472668725No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA798290.10802173235578959No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCCTGTAGGCACCATCA797120.10786341216280675No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA792570.10724772252342903No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCAT776930.10513137396082077No Hit
TTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTGTAGGCACCATCA773850.10471459943570352No Hit
TTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGATC764170.10340473664247796No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG761870.10309350891268264No Hit
TCGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATC761490.10304208867906427No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAATA744320.10071870601793997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)