FastQCFastQC Report
Fri 7 Jul 2023
SRR7277905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7277905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72072097
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA26624483.6941453222874863Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT18387142.5512147925985835No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT8809061.2222566522519804No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG7834481.0870337240222108No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA7623241.0577241841596479No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG7087530.9833944473684455No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT6890060.9559954943450584No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA6609730.9170997202981342No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG6340150.8796955082353162No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT4208310.5839028105426154No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA3962590.5498091723347526No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC3790610.5259469555880967No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA3391550.47057739973904184No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC3304150.4584506539333801No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA3113840.4320451505663836No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT2996010.4156962437210617No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA2744200.38075761830545873No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2728970.3786444565363486No Hit
TTTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2613900.362678499558574No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2553380.35428135246293724No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC2329150.32316945072376624No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCTGTAGGCACCATCAATAGATCGGA2239150.31068195504287877No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA2233950.3099604552924275No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC2217660.3077002185741869No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA2199870.3052318569279315No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT2198820.3050861694783211No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2151090.2984636342688905No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2088120.28972654979082957No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG2027310.28128916520911No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA2026210.2811365402618991No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT1964150.2725257182401672No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA1951320.2707455563558807No Hit
CCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1947150.2701669690559996No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG1863110.2585064230890909No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT1844860.25597423646491096No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1792530.24871345147623497No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1767470.24523637767886788No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC1761280.24437751547592684No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC1751640.24303996593855176No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA1713920.23780631774873986No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA1700130.23589295591052387No Hit
GCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCACGTCTGTAGGCACCAT1674560.23234511963763174No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG1606190.2228587854187176No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC1596880.2215670233655058No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGAT1567350.21746973728265462No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG1564220.21703544993286378No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1513350.20997723987412217No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1487160.20634337863098393No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATC1475690.20475191668143083No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1456980.20215590507932632No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAATA1443660.20030775571855503No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT1394600.19350068307295126No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG1389790.1928332958037838No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1369420.19000695928134295No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATA1359210.18859032227132227No Hit
TTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1327280.18416003630364744No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCG1325930.18397272386843413No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATAGATC1307800.18145718723849535No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1250350.1734860024955289No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATAG1220700.16937206641843652No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC1213290.16834392927404346No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAG1162370.16127878171770135No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1131300.156967820708755No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCAT1119040.15526674629711412No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGA1100220.15265547220028855No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG1069350.14837226118174415No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1020000.14152495105005755No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTCTGTAGGCACC1009250.14003338906595156No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATA993480.13784530232275607No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA973870.1351244157638427No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA951740.1320538793258645No Hit
TTGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA932700.12941208023959674No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATAGA905060.12557703156604422No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCTGTAGGCACCATCAATAGA884730.12275624504168375No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGA876340.12159213294432102No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC874470.12133267053406258No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA874220.12129798304606013No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG871540.1209261331746737No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA865630.12010612095829541No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGAT864870.12000067099476792No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG852700.11831208407880792No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC842770.11693429705535001No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT823550.1142675229777205No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA806680.11192681128731415No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT801370.1111900490421418No Hit
GCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCACGTTCTGTAGGCACCA789590.10955557460746562No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGA789130.1094917496295411No Hit
CCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCA789100.1094875871309808No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATC781640.10845251248898724No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC771210.10700535048952439No Hit
GCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG766540.10635738821363834No Hit
TCTAGACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATC764150.10602577582833478No Hit
TGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA763560.10594391335664896No Hit
TGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTCACGTCTGTAGGCACCA746680.10360181416672254No Hit
GGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT726850.10085040261836699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)