FastQCFastQC Report
Fri 7 Jul 2023
SRR7405200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7405200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24857455
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG968300.3895410853605086TruSeq Adapter, Index 5 (100% over 49bp)
AACACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCAC618890.24897560912812675No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG433050.17421332956249946No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCAGATCGGAAGAGCA398310.16023764299281645No Hit
AGGGCGCGCCGAACCACCTCACCCCGAGTGTTACAGATCGGAAGAGCACA351370.1413539720779943No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACAGATCGGAAGAGCAC311240.12520992193287686No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAGATCGGAAGAG298260.11998814842468788No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCAGATCGGAA262470.10559005336628388No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCAGATCGGAAGAGC259190.10427052970627927No Hit
AACACGGACCAAGGAGTCTAACGCGTGCGCGAGTAGATCGGAAGAGCACA254760.10248836817767547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)