FastQCFastQC Report
Fri 7 Jul 2023
SRR7405201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7405201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45017080
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC880467619.558523120557798No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG13554263.010914968274264No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA10797082.3984407695923418No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG7848371.7434204972868077No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG6006181.3342002635444148No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG5928211.316880170815166TruSeq Adapter, Index 6 (100% over 49bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3013100.6693237322367421No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT2113410.46946847729795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1749630.3886591489274738No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1627850.36160719442487166No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1622690.36046096281677975No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1458070.32389262031211263No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1437220.31926104491895074No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1348190.29948410692119526No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1303650.2895900844746039No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT1266390.2813132260022196No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1249530.277567980864152No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG1217640.27048400296065406No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1136570.2524752827149162No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1134650.25204877793050995No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1077430.2393380468035688No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1017770.226085299179778No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG846330.18800197613883443No Hit
TTGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC783580.17406282237763976No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT762780.16944235387990514No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC756430.16803177816064482No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA753270.1673298223696428No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG748960.16637240798381414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT706940.157038173066756No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA704210.1564317365764283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGT703220.15621182004696882No Hit
GTTGAATCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC702050.15595191869397126No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC676810.1503451578822971No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT666330.14801715260074622No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG650560.1445140377829926No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCAC636170.141317473279031No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT630300.1400135237558722No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC623150.13842523770977594No Hit
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT614360.13647264549366597No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT546720.1214472373596866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC540980.12017216576463866No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGAA483280.10735480844159594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC475720.10567544585299625No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC474700.10544886518628041No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT473210.10511787970254845No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA461270.10246555307452195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGAGATCGGAAGAGCACACG454710.10100832839446718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)