FastQCFastQC Report
Fri 7 Jul 2023
SRR7405203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7405203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23769205
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC327933013.796548938006131No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG17576867.394803486275624No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5458422.296425143373537No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG5367142.2580225127428535No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC3729801.5691732222428139Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA3273911.3773746324288085No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT2867301.206308751176154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2719981.1443293959558176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2431571.022991723955429No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2357220.9917117547684073No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2354340.9905001029693673No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGT1988150.8364394181462947No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1710860.719780068369977No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1552390.6531097695526628TruSeq Adapter, Index 8 (100% over 49bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1346690.5665692226559533No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1148890.4833523039579994Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG1103970.46445390159241756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT812120.34166897883206443No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG767180.3227621622178781Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT620210.26093005634811933No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA580650.24428667260852854No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG554880.2334449132817021No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGAA550310.23152225747558658Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA513150.2158885835685291No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG507980.21371350030428027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC505860.21282158995220915No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC497250.2091992559279959No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA476170.20033063789891165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT456880.19221509511992513No Hit
AGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAGATCGGAAGAGCA441620.18579502343473414No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC439040.18470958536476084No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT424450.17857139100781871No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT412220.17342607798620105No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT401090.1687435486378278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACACGTCTG400070.16831442195900115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC396310.16673254322136563Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG368770.15514612289304586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC362670.15257977706869036No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA357090.1502322017080504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTC341910.14384578701727718No Hit
AGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAAGATCGGAAGAGC339080.14265517083974832No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC336260.14146876178652168No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAGATCGGAAGAGCACACGTCTGA334230.14061471555317057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG332120.13972701232540172No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC330530.13905807956134839No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC326890.13752668631533954No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC324490.13651697648280622No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAAAGATCGGAAGAGCACACGTCT314320.1322383310674463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC308410.129751920604833No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC299440.12597813010573974No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGAGATCGGAAGAGCACACGTC288620.1214260216107354No Hit
ACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTG264480.11127002354517117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG264020.11107649582726895No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAGATCGGAAGAGCACACGTC261440.10999105775729562No Hit
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT254820.10720594146922457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAA249050.1047784307468424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT241450.10158101627715356No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT239710.10084897664856692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)