FastQCFastQC Report
Fri 14 Jul 2023
SRR7405204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7405204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141832207
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG24489491.7266522546603256TruSeq Adapter, Index 9 (100% over 49bp)
GTGGGGGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGTAGATCGGAAGAG20541511.448296577659544No Hit
AGGGCGCGCCGAACCACCTCACCCCGAGTGTTAGATCGGAAGAGCACACG7843290.5529978109978928No Hit
AACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAGATC7726200.5447422812788917No Hit
GTGGGGGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGTCCAGATCGGAAG7425560.5235454031960456No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTAGATCGGAAG7069660.49845237196372466No Hit
GTGGGGGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGTCAGATCGGAAGA6942710.4895016545854074No Hit
AGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATC5763510.4063611588586505No Hit
AGGGCGCGCCGAACCACCTCACCCCGAGTGTAGATCGGAAGAGCACACGT5324960.37544081930558976No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCAC4851700.34207322177536165No Hit
AAGGGCTGGGTCGGTCGGGCTGGGGCGCGAAGCAGATCGGAAGAGCACAC4831550.3406525289421746No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACGT3510570.24751571411421383No Hit
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCAGATCGGAAGAGCACA2987720.21065173159154185No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGAGATCGGAAGA2929300.20653277996301642No Hit
CGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGAGATCGGAAGAGCACAC2731890.19261422054865154No Hit
ACTTCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGAAGATCGGAAGAG2630200.18544448088578358No Hit
GATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAGATCGGAAGAGCA2619030.18465693056584814No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACAGATCGGAAGAGC2433690.17158937673443944No Hit
TGTGGGGGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGTAGATCGGAAGA2403510.16946151024780995No Hit
AAGGGCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTAGATCGGAAGA2313230.1630962423083496No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGAGATCGGAAGAG2226190.15695941331576402No Hit
GACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAGATCGGAAG2139980.15088110417685313No Hit
AGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAGATCGGAAG2082090.1467995206476622No Hit
AGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAT2049300.1444876338982725No Hit
GTGGGGGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGAGATCGGAAGAGC2027590.14295695194251615No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGTCCAGATCGGAAGAGC2003640.1412683368876859No Hit
AGCCCTCCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAG1976830.1393780751081452No Hit
AAGGGCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCAGATCGGAAGAG1939800.1367672435640799No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG1827870.1288755240197313No Hit
AACACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCAC1787970.12606234069247754No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAAGATCGGAAGAGC1781520.1256075779741621No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGAGATCGGAA1762150.12424187970225974No Hit
AACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTAGATCGGAAGA1740070.12268511058281706No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGTAGATCGGAAGAGCAC1700120.11986840196317329No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAG1665400.11742043892752795No Hit
AAGGGCTGGGTCGGTCGGGCTGGGGCGCGAAGAGATCGGAAGAGCACACG1636460.11537999969217147No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCA1632460.11509797630096809No Hit
GTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTA1623800.11448739565901277No Hit
GGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGTAGATCGGAAGAGCACAC1605270.1131809222992631No Hit
GTGTGGGGGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGTCAGATCGGAA1546180.10901473175271115No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACG1519590.10713998125968667No Hit
GTGTGGGGGGAACCTCCGCGTCGGTGTTCCCCCGCCGGGTAGATCGGAAG1435560.10121537486898163No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)