FastQCFastQC Report
Fri 14 Jul 2023
SRR7405205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7405205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110873697
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC69121606.234264922184384No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG29745922.6828653508324884No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG28049922.5298985024374177TruSeq Adapter, Index 10 (100% over 49bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG25015772.256240269502333No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT19747011.7810364887535048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG16757761.5114279088213323No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCAGATCGGAAGAGCACA16289231.4691699150250217No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGT14178891.2788326161794714No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC13482781.2160485637995817No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT13394941.2081260355194974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA12473081.1249809772285306No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT12083341.089829267621517No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGAA10838930.9775925483931504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCT10451480.9426473801085572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10164450.9167593644866013No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC7470110.6737495187880315No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA7369150.6646436620580984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCAGATCGGAAGAGCACAC7235500.6525894054024374No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG7053080.6361364499282459No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT7024720.6335785844680547No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC6381190.5755368651592813No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT6057200.5463153267090931No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG5562390.5016870683043968No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT5462920.4927155987231128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA5183960.4675554383290746No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG5157360.4651563120511802No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATGAGATCGGAAGAGCACACGT5057890.4561848424698962No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG4993940.45041701820405605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCAGATCGGAAGAGCACAC4762000.4294977193734236No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC4201530.3789474071564512No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT3588130.3236231944173378No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT3566990.32171652037543225No Hit
AGGCCGAAGTGGAGAAGGGTTCCATGTGAGATCGGAAGAGCACACGTCTG3491390.3148979509540482Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA3484740.3142981693845746Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC3366600.3036428017729038Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG3362110.3032378364726126No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG3346050.3017893414341546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT3173440.2862211765158332No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCTG3146490.2837904827869138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2792710.2518821032909185No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2749880.24801914921263965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCT2731580.2463686224876221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCAGATCGGAAGAGCACAC2714810.2448560906199421No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT2707350.24418325294952506No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACAAGATCGGAAGAGCACACGTC2691680.24276993307078054No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACACGTCT2668200.24065220807059406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2636270.23777235460994864No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACAC2540360.22912197110194674No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCAGATCGGAAGAGCACACG2534500.2285934417790723No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTAGATCGGAAGAGCACACG2514630.2268013124880286No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC2493150.22486397292227028No Hit
GAGCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTC2482500.22390342048394038No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACG2421500.21840166473388184No Hit
GTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACACGTC2357840.21265999635603386No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTAGATCGGAAGAGCACACGT2325710.20976210435194564No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCAGATCGGAAGAGCACAC2261140.20393836060143283No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2236450.20171150241341732No Hit
AACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACG2222250.20043076582897745No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG2120180.19122479518293686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGCA2060680.1858583285086994No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA2011780.18144790463693114No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1993080.17976130082502795No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1972740.17792678095689368No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCACACGTC1898960.17127236228083925No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1820140.1641633723100259Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACACGTCTG1790650.1615035890793828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACAC1698250.15316978200880232No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC1695510.15292265396363575No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATAGATCGGAAGAGCACACGTC1693910.15277834561609324No Hit
GCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTG1693610.15275128780092903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1651560.14895868404207718No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1644120.14828765022600446No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCAGATCGGAAGAGCACACGT1594700.1438303261412849No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1594260.1437906413457107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA1555420.14028755620911604Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCCGAAGTGGAGAAGGGTTCCATGTGAGATCGGAAGAGCACACGTCT1520380.13712720339793485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTC1504290.1356760025779604No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT1494740.13481466212856597No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC1486820.1341003358082305No Hit
TCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACG1485910.1340182604355657No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC1476480.13316774311223697No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTAGATCGGAAGAGCACACG1451940.1309544138318036No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1437530.12965473677674877No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGAGATCGGAAGAGCACACGTC1392810.12562131846293534No Hit
GTGGACGGTGTGAGGCCGGTAGCGGCCCCAGATCGGAAGAGCACACGTCT1373700.12389773563697438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1369750.1235414744039788Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1358220.12250155237450051No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATAGATCGGAAGAGCACACGTC1345270.12133355668657825No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC1328020.11977773231463547No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCAGATCGGAAGAGCACACGTC1301970.1174282120312088No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCAC1301590.11739393879866747No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT1300170.11726586514022347No Hit
ACCTATTCTCAAACTTTAAATGGGTAAGAAAGATCGGAAGAGCACACGTC1277240.11519774613450474No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAAAGATCGGAAGAGCACACGTCT1260660.11370235088309537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACA1240500.11188406570405965No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCACACGTCT1233750.11127526486286463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1232050.1111219372436007No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCAGATCGGAAGAGCACACG1225180.11050231327634003No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTAGATCGGAAGAGCACACG1220480.11007840750543386No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCAC1209650.10910162037800543No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAGATCGGAAGAGCACACGTC1186960.10705514762441806No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTC1182160.10662222258179054No Hit
CCATCTAAGGCTAAATACCGGCACGAGACCGAAGATCGGAAGAGCACACG1173500.10584115365071664No Hit
TGGAGCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACG1153730.10405804363139437No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT1147450.10349163336728999No Hit
TCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAA1140790.10289094987064426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTATTCTCAAACTTTAAATGGGTAAGAAGAGATCGGAAGAGCACACGT1110670.10017434522815631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)