FastQCFastQC Report
Fri 7 Jul 2023
SRR7451184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76252779
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG40603555.324861668320311TruSeq Adapter, Index 4 (100% over 49bp)
ACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12615611.6544459317345013Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA11237041.47365645519621No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC7759191.0175616025745107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC5786910.7589113571847657Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC5682050.7451597272277775No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC5041850.6612021314003519Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG4645350.609204026518168No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT4385740.5751580542395708Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC4328520.5676540654341267No Hit
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC4187170.5491170361148412Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG4159440.5454804473421224No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC4150310.5442831139308378No Hit
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC3960450.5193843492576186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG3895010.5108023669537342Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA3882950.5092207852516432No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC3558310.46664659920132223No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA3462010.45401755128163923No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT3088550.4050409756213606Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC2818170.36958259580283626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG2762040.36222155260728267No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG2668470.3499505244261327No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG2637560.3458969016722656No Hit
GCCCACCCAGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2621460.34378550321425005Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCCGGACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA2595660.3404020199709705TruSeq Adapter, Index 4 (100% over 36bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2542540.3334357164871329Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG2433220.31909918981444596No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC2400910.31486196719466447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT2371800.3110444014112587Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA2365610.3102326277183944Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG2326570.3051128143146101TruSeq Adapter, Index 4 (100% over 35bp)
TAAACGATGCCGACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2058720.2699862256823453Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT2003760.2627786195175916Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1831790.24022599884523554TruSeq Adapter, Index 4 (100% over 35bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1819070.2385578629206419No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA1746850.22908673269468643No Hit
AAACGATGCCGACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1693990.22215452632880434Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1596780.20940613849627696Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCCAGGAACGGTGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1596500.2093694185230941Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1552420.20358864560201798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1535910.2014234786118418No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1526440.20018155666169227Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC1513800.19852391215800805No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1502140.19699478756046387No Hit
TCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1479330.19400342117367292Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG1418510.1860273184273061No Hit
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1403360.1840405055925896Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1359900.1783410411835613Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1336740.17530377483029175No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTAGATCGGAAGAGCACACGTCTG1292690.16952693619205667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA1268960.1664149184648077TruSeq Adapter, Index 4 (100% over 39bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT1261940.16549429628000836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1232520.1616360762405787No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1229100.16118756799670214No Hit
ACAGCCCCCCCGGCAGCAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1217620.1596820490962041Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGTGGCCGAGCGGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1210860.1587955240293603Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA1210240.1587142155173125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAA1208300.15845979856025968No Hit
TGGGAAACTCGACTGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1195270.15675100837964215Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT1191210.15621856876849038No Hit
CGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACACGTCTGAACTC1142520.14983322771751048Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCACCCAGGGACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1137580.14918538247635538Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1131070.14833164309985344TruSeq Adapter, Index 4 (100% over 50bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC1129980.14818869748996294Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTAGCGTGGCCGAGCGGTCTAGATCGGAAGAGCACACGTCTGAACTCCA1128300.14796837765086568Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCAC1124980.1475329836831258No Hit
ATCTGAGGGTCCAGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1097100.143876723496202Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1090550.1430177384092454No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1061070.1391516498041337Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1011160.1326063145842855Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1007960.13218665774790975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC999200.1310378471583311Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAAACGATGCCGACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT997500.13081490446400648Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCCGGACGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC986360.12935397410237337TruSeq Adapter, Index 4 (100% over 37bp)
CGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT959520.1258341023872717Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGACGAGGCGCCGCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT957320.12554558831226337Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA949740.12455152618109826No Hit
GGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAACT933820.12246373342012885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGAGACCGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC910120.11935564997572089Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT903100.11843502779092156No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT902740.11838781639682928No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC870270.114129610935229No Hit
CGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC863460.11323652873031684TruSeq Adapter, Index 4 (100% over 37bp)
ATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAA860470.11284441187382824Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG834150.1093927343946376Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCACTCTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA831240.1090111089590584TruSeq Adapter, Index 4 (100% over 36bp)
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCAC827190.10847998077552032No Hit
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT797020.10452340366506511Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTTCCAGGCACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA781770.10252347655421187Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCTCCCACGGCGCGACAGATCGGAAGAGCACACGTCTGAACTCCAGT781310.10246315088398286Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGGGCGGGGCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC780930.10241331663466324Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC770720.10107434904110184TruSeq Adapter, Index 4 (100% over 38bp)
GCGCAGCGGAAGCGTGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA765000.10032421244608015Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)