FastQCFastQC Report
Fri 7 Jul 2023
SRR7451185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70626776
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC25792323.651918077076037Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC13061071.8493085398659566No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA11614891.6445448394812754No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG9720531.3763236198124065No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA8815021.248113038601677No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG8705031.2325396249150606TruSeq Adapter, Index 6 (100% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC7453781.0553759384401178Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT5804210.8218143781616196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG5530060.78299765516693No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC5016490.7102816076441038No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC4476360.6338049467244548No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG4452680.6304521106839139No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA4440930.6287884357060274No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG3969110.5619837439556918Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG3929520.5563782212004128No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT3679190.5209341567566386No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC3438830.48690173823027116No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT3354910.47501955915416555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG3076620.4356166562098205No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2948390.4174606526000847Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT2882740.4081653111278929Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2861400.4051437941893312No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2831150.4008607160547722Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2790270.3950725430253251Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA2744700.38862031589832163No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA2685030.38017167879785424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC2634450.37301008897815185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG2308200.32681656033683315No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA2274800.32208747571884067No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2251490.31878702774143336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2249340.31848261061782007No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2096520.2968449246501072No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1990370.2818152141052No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1979690.28030303974232096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC1974760.27960500419840767No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1959050.2773806353556334No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1913130.27087885195269285No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG1894490.26823962628564557No Hit
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC1880330.2662347209505925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1819410.25760909715034985No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1794600.2540962651332124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC1717800.24322220229902608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1686690.23881735731502174No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA1627870.23048907117040143No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1582380.22404817119218357No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1525910.21605262004314058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1523310.2156844877076082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1482500.20990622593334857No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1445780.20470706464075322No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT1375870.19480855249572768No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT1345720.19053963329715065No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1321980.18717830189502066No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1271160.17998273062896145Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1254690.17765075387272386No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG1220180.17276450506533103No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG1208350.17108950293865885No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC1193020.1689189380526162Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1191600.1687178811616716No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1189290.1683908097404871No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1147970.16254033739271917No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC1125600.15937298341354278Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC1095490.15510972778935853No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1050130.14868723442791726No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG1003710.14211465634506665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1002680.1419688193044519Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA994380.14079362761794478Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC994180.14076530974598075No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCAC980510.13882978319723951No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC896460.12692919750435727Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA891520.1262297460668458Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC875780.1240011295432769Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAA871350.12337388867927372No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT859710.12172578853096734No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC857490.12141146015216665No Hit
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT842800.11933151245640888Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT837280.11854993919020174No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT835560.1183064054913111Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC812900.11509799059778687Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA811260.11486578404768186Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT807630.11435181467153478No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG807550.11434048752274915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT794200.11245026956915039Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT784090.11101880114136882No Hit
ACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCACACGTCTGAACTCCA766310.10850134232376683Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCAGTGCGCCCCGGGCGGGTCGCGCAGATCGGAAGAGCACACGTCTGAAC765020.10831869204959887Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTAGCGTGGCCGAGCGGTCTAGATCGGAAGAGCACACGTCTGAACTCCA764300.10821674771052836Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC763950.10816719143459132No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC761290.10779056373746976No Hit
ACCCCTCCCACGGCGCGACAGATCGGAAGAGCACACGTCTGAACTCCAGT758920.10745499695469604Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA753540.10669324619886372Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGCAGATCGGAAGAGCACA749200.10607874837724435No Hit
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC748880.1060334397821019Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG709880.10051145474911669No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT708890.10037128128289476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)