FastQCFastQC Report
Fri 7 Jul 2023
SRR7451188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81424599
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC52025236.389375033950122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC19035592.3378180836972864No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC13166421.6170076563717557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG10162041.2480307087542428TruSeq Adapter, Index 12 (100% over 49bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC9640171.183938283810277No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT7941130.9752740692035831No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC7391180.9077330549702799Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA7279340.8939976480571922Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT6855070.8418917727798696No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA6458110.7931399207750474No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC6240800.7664514258154345Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA6139950.7540657338699328Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT6008860.7379661765359139Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGT5870870.7210192094406261No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG4635010.5692395242867576No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC4114900.50536325023842Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA4077590.5007810968771244No Hit
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC3947690.48482768702367207Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT3894570.4783038599919909No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3869220.4751905502168945Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT3650700.4483534515165374No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3625120.44521189474939876No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT3613000.44372340108177877No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3591550.4410890620413126No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC3578930.4395391618692528No Hit
CTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAACTCCA3556180.4367451659172432Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT3532600.43384923516786367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT3489490.42855476635506673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2831500.34774503464241807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTC2682170.32940536802643633No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG2660600.32675629142490464No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGATCGGAAGAGCACACGTCTGAA2629340.32291715676733024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2496440.3065953078872393No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2464050.30261739452963105No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA2425870.2979283938506102No Hit
GTCTAGGGGTATGATTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG2409330.2958970666837426Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC2225870.2733657920747022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA2220230.27267312670462157Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2135850.2623101650153659Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC2087240.25634022465373146No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2047490.25145840755076976No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG2007330.24652623711416743No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC1950930.23959958341336135Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGGAGATCGGAAGAGCACACGTCTGAACTCCA1942860.2386084824317035Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA1940910.23836899706438838No Hit
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1935970.2377623008005234Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1920380.23584764599209143No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1916120.23532446257426456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1914960.23518199948396432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAGAAGGTTGCGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1909880.23455810939885624Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAGATCGGAAGAGCACACG1810780.22238734021889378No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1791070.2199666958138781No Hit
GTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACACGTCTGAACT1777610.21831363271435944Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT1765430.21681777026620663Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG1698650.20861631753323095No Hit
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1666650.20468630124908568Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACG1646490.20221039099007415No Hit
CATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1596640.19608816249742905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1596240.19603903729387726No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA1507840.1851823673089259Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1496960.1838461617723165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCAATGGATAGCGCATTGGAGATCGGAAGAGCACACGTCTGAACTCCAG1493330.18340035055008377Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGGGGTATGATTCTCGGTAGATCGGAAGAGCACACGTCTGAACTCC1470720.18062354841931735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1464770.1798928110164841No Hit
TGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAAC1447300.17774726775135855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTATAGTGGTGAGTATCCCCGCCAGATCGGAAGAGCACACGTCTGAACTC1398320.17173188657643865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1395640.1714027477126415No Hit
GTATAGTGGTTAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1377240.16914298834925795Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC1307380.16056327154893327No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT1266730.15557092273797996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1244870.1528862303638732Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1228830.15091630970144537Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGAGATCGGAAGAGCACACG1200660.14745666724130874No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1194570.14670873601723233No Hit
ACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCTGA1186780.14575202267806073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGCAAGATCGGAAGAGCAC1155380.14189569419924317No Hit
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA1150960.14135286069999559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC1149480.14117109744685385No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1146140.1407609019971962No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG1144170.14051896036970352No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA1138790.13985822638193157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT1111650.13652508132094085No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA1101640.13529572310205668No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1099950.13508816911705024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCAGCGGAAGCGTGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA1092040.13411671821681306Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC1091090.13400004585837752No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC1087270.13353090016445768No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG1061400.13035372762474395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1057350.12985633493878185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC1055870.12967457168564012Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC1031750.12671232191146561No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG1031730.126709865651288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1023860.12574332727140602No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1000350.12285599343264803No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT953340.11708255388522086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC946570.11625110981510636No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG944530.11600057127699209No Hit
GCGCAATGGATAGCGCATTGGAGATCGGAAGAGCACACGTCTGAACTCCA942630.11576722656012099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA935690.11491490427849697Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC933040.11458944980496617No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAGATCGGAAGAGCACACGTC932890.11457102785363425No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT894670.10987711465425823No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC892810.10964868245774229No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC867830.10658081349593138No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC863280.10602201430552946No Hit
ACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAA858130.10538952730979982Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA853020.10476195283442538No Hit
ATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAACTCCAG845500.10383839900765124Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC842550.1034761006314566No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCAGATCGGAAGAGCACA827290.10160197411595481No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC817400.10038735345813615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)