FastQCFastQC Report
Fri 7 Jul 2023
SRR7451189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77940885
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1197205115.360424762946945TruSeq Adapter, Index 2 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT8437051.082493482079399Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA7659140.9826857880815698No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG7414160.9512542743131541No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC6898200.8850553852448558Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4972080.6379296308991103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT4909000.6298363176143048Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC4907450.6296374489460826No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT4453780.5714305142929799No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC4438190.5694302803977656No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA3742940.4802280600226697No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC3005840.38565638560557786No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG2998770.384749287873752No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC2984730.3829479226467598Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC2970070.3810670099524787Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG2941600.3774142415755223No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2916090.3741412482036867TruSeq Adapter, Index 2 (100% over 50bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG2898200.37184591886530416No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2882740.36986236427774716TruSeq Adapter, Index 2 (100% over 50bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2809330.3604436875460164Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG2763480.35456102403764084No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG2624380.33671416484429195No Hit
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT2374500.30465396947956136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC2371880.30431781727908275No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG2341210.3003827836956175TruSeq Adapter, Index 2 (100% over 50bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2281560.2927295475282325No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2232500.28643503342308724No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2062540.2646287631966201No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2040100.2617496580902308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1888000.24223486813114836No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1884720.24181403636871715Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1796600.23050803182437563No Hit
CGGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACA1753090.22492559585383204No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1744870.22387095040042204No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC1713660.2198666335389956Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1620940.2079704381083176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1492160.19144765933822794No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1471320.18877383801839046No Hit
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC1445470.18545722184191263Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA1416260.18170950971367594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCATCGACCTGGTATTGCAGTAGATCGGAAGAGCACACGTCTGAACTCC1375400.17646707501460884Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1335280.17131958406682193Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1313240.16849179990707058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1309740.1680427416239885No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1300010.16679435959702024No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACA1266780.1625308719550721No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1256540.16121705572114045No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1248050.16012776862874986TruSeq Adapter, Index 2 (98% over 50bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1238860.15894866987974282No Hit
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1221560.15672903893765128Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGCGGGGCGCGGGACATGTGGCGTAGATCGGAAGAGCACACGTCTGAAC1193550.15313528964932846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1182290.15169060500147002No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1181820.15163030288917043No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1152560.14787617564260402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACAC1146380.1470832670170476No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA1133370.1454140532276481No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC1113720.14289291172405855No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACA1106390.14195245537691803No Hit
GGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGT1005970.12906833172345938No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC994950.12765443964358372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT966880.12405299221326521Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT962910.12354363181788353TruSeq Adapter, Index 2 (100% over 50bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAA950200.1219129087384625No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG928300.11910308691003445No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT915870.11750828849325999No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC914500.11733251425102499No Hit
CGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACACGTCTGAACTC890520.11425582350007958Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCAC883660.11337566926523865No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT861910.11058509279179983No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA828150.10625360489555642Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG824300.10575964078416611Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT817510.10488846771498682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGCGGAAGGATCATTAACGGAGCAGATCGGAAGAGCACACGTCTGAAC801100.1027830258791647Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC789690.10131909587631704No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG780500.10013999712731002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)