FastQCFastQC Report
Fri 7 Jul 2023
SRR7451192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72088070
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC23115283.2065333417859567Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC12633631.7525271518574432Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG11929241.6548147287061505TruSeq Adapter, Index 11 (100% over 49bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA10326891.4325380052483025No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC9181941.2737114476778195No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA8660301.201349959847725No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7432331.0310069336021896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA5969930.8281439633492754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG5813100.8063886299078336No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG5075330.7040457595826882No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG4912600.6814719828121352No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT4811220.6674086294722553No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC4560910.6326858244366925No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC4367000.6057867827505994No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC3949830.5479172906141058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA3927460.5448141419238995No Hit
AGTACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG3619540.5020997232967952Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC3508730.4867282478224206No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG3339660.46327499127109384No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG3298960.4576291194923099No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3238470.4492379945807954Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC3084050.42781697443141425Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2661910.36925804782955074No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2635750.36562915333979673No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC2634290.3654266232956438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2608460.3618435061446367No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT2515250.34891348873676326No Hit
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC2511810.34843629466012893Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2488380.3451861036090993No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG2483430.3444994435278958No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2471930.3429041726321706No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA2420540.33577539251640387No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT2399690.33288309702284996No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG2264660.3141518423228698No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC2230510.3094145813586076Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC2091160.290084059678668No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC2074950.28783542131173717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC2073360.2876148577705021Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2068830.2869864597567947No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT1983630.27516758320759593Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1937700.2687962099692779No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1865780.2588195245066209No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT1844840.25591474428431776No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1844310.2558412231039061Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1755050.24345914656891215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1724020.23915468953462066No Hit
CGCATCGACCTGGTATTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1676550.2325696887154837Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCAC1664710.23092725328892838No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1628270.2258723253376044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1570060.21779748022106846No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1544980.21431840247630435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1514830.21013601834533788No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT1497370.20771398096800206No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1479280.20520455048942216No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT1464590.20316676531914368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCATCGACCTGGTATTGCAGTAGATCGGAAGAGCACACGTCTGAACTCC1444640.20039931711308126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC1431990.19864451912778355No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC1349300.18717382779147784No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA1322020.18338956778840104No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA1303930.18088013730982117No Hit
ATCTGAGGGTCCAGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1262850.17518155223187415Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC1233090.171053268592154Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1193580.16557247267127556No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1160450.16097670529950378No Hit
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC1159030.1607797240236838Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1152820.1599182777399922No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1141790.15838820487217928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1139690.15809689453469902Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACACGTCTGAACTC1122780.15575115272194137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT1105910.1534109596775167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC1093590.15170193903096588Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC1092000.15148137548973084No Hit
CTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAACTCCA1080110.1498320041027593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA1061340.14722824456251915Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1055550.1464250603463236No Hit
ATCAGAAGGTTGCGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1037630.14393921213315877Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1027130.14248266044575753Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1023720.14200962794537292Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1015260.1408360634429525Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC1014520.14073341122879277No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1008280.13986780336885146Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC1001670.1389508694018303No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC985370.13668974630615024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGAACTCC967920.13426909612089766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC950030.13178740948398257No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA944820.1310646824086149Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG943470.13087741147737758No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC940030.13040021740074328Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC939640.13034611690949693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC907000.1258183219498039No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC892470.1238027318528572No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC877460.12172055653591501Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG869650.12063715951890513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC851590.11813189061657499No Hit
CGGCGGGTGCGGGGGTGGGCGGGCAGATCGGAAGAGCACACGTCTGAACT850760.11801675367366611Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC844510.11714975862164155No Hit
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT842940.11693196946457299Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCAGAAGGTTGCGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC810990.11249989075862343Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA807810.11205876367615333No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG799900.11096149473831106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC789400.10950494305090981No Hit
GCGCGCGGGTCGGGGGGCGGGGCGAGATCGGAAGAGCACACGTCTGAACT751850.10429603677834627Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC751390.10423222594251726Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT737280.10227489791306661No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC733250.10171585950352119No Hit
TCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACT733170.10170476196685527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA722980.10029121323403443Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)