FastQCFastQC Report
Fri 7 Jul 2023
SRR7451193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67580663
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC18645522.759002231155974No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG16394902.425975015959817TruSeq Adapter, Index 1 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC15343432.2703876107282346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC7148261.0577374773609427Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA6843211.012598825791336No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG6199300.9173186122781898No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG5943550.8794749468498111No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3969630.5873913962637508No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3740630.5535059636807648No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA3737910.5531034816867659No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT3468650.5132607237073126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC3189260.47191901624285637Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG3149000.465961690846389No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC3114490.4608552005475294No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT3083120.4562133401976243Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2972470.43984031349322517No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2729080.4038255735963999No Hit
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC2682070.39686944178100175Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG2636160.390076078419059Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2443130.36151317426406426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT2360630.3493055402549099Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC2282780.33778597288990786Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA2191310.32425103612848544Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT2121860.3139744278625973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1802650.2667405023830559Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1801860.2666236050392107Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC1694780.25077883595193495Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1666740.2466297201020357No Hit
GCGCGCGGGTCGGGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAACTC1636140.24210179766954937Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1626070.2406117264638259Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1554850.23007320895919592Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1545500.2286896771048251Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1422490.2104877248688726No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTAGATCGGAAGAGCACACGTCTG1406590.2081349808598356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT1386120.20510600791235212No Hit
AGTACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG1366750.20223980341832395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1365350.20203264356847164No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT1334230.1974277760488973No Hit
GTGGGGGGGCCGGGCCACCCCTCCCAGATCGGAAGAGCACACGTCTGAAC1319870.19530290787469784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA1247720.1846267770412374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAAACGATGCCGACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1210430.1791089264690996Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC1205620.17839718441353555No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG1193990.1766762779465481No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC1186890.17562568156515423No Hit
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1144050.16928659015967334Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1115020.16499098270166423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC1099510.1626959475079432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1092720.16169122223615948No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG1077180.15939174790279875No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC1062560.1572284071850553No Hit
GATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA1042280.15422754878862316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA1006230.148893182654926No Hit
GCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACAC1005210.14874225190717646No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1003570.1484995789402066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTAGCGTGGCCGAGCGGTCTAGATCGGAAGAGCACACGTCTGAACTCCA998960.1478174311489072Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA978890.1448476467299529No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG962820.14246974759629097No Hit
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT944450.13975151442358594Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC931080.13777313785749631Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC925990.13701996383196183No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT922800.13654793531694118No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT921460.13634965374636826No Hit
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC865510.12807065831834175Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC860840.12737963224776294Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC842520.12466879764112405No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT841650.12454006259157299No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC818540.12112044535579652No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA809280.11975023092034479Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT809130.11972803522214631Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCAC797760.11804560129870287No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCAGATCGGAAGAGCACACGTCTGAAC786520.1163824036470314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAA773450.11444841847733869Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC769820.11391128258093591No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT768290.11368488645931159Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC768210.11367304875360573No Hit
CGGCCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCTGA756950.11200689167550781Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG756230.1119003523241552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTGAGGGTCCAGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT728130.10774235819497659Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA725030.10728364709887502Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGAAGGTTGCGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT716960.10608951853579773Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC714820.10577285990816633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT709380.10496789592016875No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC702750.10398684605979672Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG701860.10385515158381918No Hit
CTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAACTCCA686720.10161486577898769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTCTGA684200.10124197804925353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATGCCGACCGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTCTGA681360.10082173949669598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG679470.10054207369939534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)