FastQCFastQC Report
Fri 7 Jul 2023
SRR7451194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67740044
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG977273914.426827062586495TruSeq Adapter, Index 3 (100% over 49bp)
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC7478881.1040559702027946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC6092940.8994591146117354Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC5895760.8703507780420101No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA5720290.8444473404829794No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA3973620.5865983789440704No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3515540.5189751574415866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT3484830.5144416499050399Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG3423510.5053893971489006No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG3388810.5002668731658928No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC3219820.4753200337454756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG3103910.45820903216419523Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC3099350.4575358705110968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2960080.43697639168938246Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG2806140.4142512809705291No Hit
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC2787330.4114744891514981Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2701580.3988158023635178No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG2693550.3976303883121186No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2462670.3635471509289247No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG2405880.35516363113079763No Hit
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2362800.348804024987052Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC2351930.34719936113416167No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG2334100.344567240021279No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG2247590.33179635962444903No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2088650.3083331330579No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG2041470.3013682719190439Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCATCGACCTGGTATTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1971940.29110403294098836Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1928230.28465142420043305No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG1730410.2554486088022027No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTAGATCGGAAGAGCACACGTCTG1660950.2451947034460149Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1653750.24413181662533315Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC1615520.23848818285385232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC1605450.23700161753659327No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1516740.22390596616677721Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1449330.2139546883081446No Hit
ACCCCTCCCACGGCGCGACAGATCGGAAGAGCACACGTCTGAACTCCAGT1416700.20913774428608284Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1396180.20610851684713993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGCGGAAGGATCATTAACGGAGCAGATCGGAAGAGCACACGTCTGAAC1386510.20468100079769655Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC1382560.20409788927801698Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1380890.20385135858488665No Hit
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1366580.20173887102878174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1363520.20128714412999202TruSeq Adapter, Index 3 (100% over 50bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1306080.19280766927166448Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAAACTCGACTGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1267760.1871507494149251Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC1237220.18264233781720013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC1223770.18065680618689883No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1181330.17439167887165824No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1170290.17276191907994626TruSeq Adapter, Index 3 (100% over 50bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA1133550.1673382438310787No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT1126780.1663388349732988No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1099040.1622437682502834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1098490.16216257550703686No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATG1091400.16111592723500445TruSeq Adapter, Index 3 (97% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1081560.15966331524673943Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA1059500.15640674812670627Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1047270.1546013167632427Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGCGCCCCGGGCGGGTCGCGCAGATCGGAAGAGCACACGTCTGAACT1044310.1541643521814069Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA1029890.1520356260766527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCAC1025730.1514215136913699No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG1013330.14959098638908472TruSeq Adapter, Index 3 (100% over 50bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1012090.1494079336588562No Hit
GTGGGGGGGCCGGGCCACCCCTCCCAGATCGGAAGAGCACACGTCTGAAC1009120.148969492845325Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGTGCGCCCCGGGCGGGTCGCGCAGATCGGAAGAGCACACGTCTGAAC986380.14561254197000523Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTAGATCGGAAGAGCACACGTCTGA952150.14055940087668084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCACACGTCTGAACTCCA948930.14008405427076487Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGATGTAGCTCAGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT948770.1400604345636386Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC904050.1334587264218488Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC837020.12356354536764104Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT804910.11882336539373964No Hit
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC798990.117949436230068Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC788150.11634920107226385No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA778980.11499549660759004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGATCGGAAGAGCACACGTC769330.11357093302153745No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC763230.11267043168734878No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC735680.10860341336654579Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAAC731630.10800553952991232Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC695620.10268962919480831TruSeq Adapter, Index 3 (98% over 50bp)
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA692850.10228071301518493Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG691700.10211094637021495No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAA690440.10192494117659562No Hit
ACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGTCTGAACTC688030.10156916933800635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)