FastQCFastQC Report
Fri 7 Jul 2023
SRR7451195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76386381
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG45664885.97814419300739TruSeq Adapter, Index 5 (100% over 49bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG18999992.487353079340151No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA11145421.4590847025466491No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC10502661.374938812718461Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACAC9596241.2562762987815852No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG7718331.0104327366942545No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT6802030.8904767984753722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA6151770.8053490582306813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC5203100.6811554536141724No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT5108440.668763192224017No Hit
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG4658460.6098547855016196Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC4496620.5886677626473755Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC4385200.5740813928598084Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAACT4131090.5408149916148011Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC4106710.5376233231942222Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC3712480.4860133379011633No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC3443770.45083560117869703No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC3411800.4466503001366173Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT3346250.4380689274963818Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT3336870.43684095990880883Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA3332280.4362400674539091Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG3309170.4332146590371915No Hit
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC3170000.4149954427085634Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC2766610.36218629077348224No Hit
CGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA2413070.3159031712734237Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2360760.3090550919017881Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCA2240250.29327871940941935Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC2183740.28588080380454206Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT2146990.2810697367636778No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2071370.27117006629755114No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA1955310.25597625838564075No Hit
GGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA1949830.2552588530146493Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA1867940.2445383556003262No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACA1837310.24052847849932832No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1831190.2397272885594619No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACAC1816880.23785391796477434No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTG1673530.2190874836706821Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1637530.2143746016714681Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG1629740.21335478637219377No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1534510.20088790435038414Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT1507880.19740168080485446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA1502870.1967458047266305No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC1479040.19362613866992862Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1469180.19233533265569946No Hit
GGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACACGTCTGAACTCC1426130.18669951126497275Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG1425260.1865856166166584TruSeq Adapter, Index 5 (97% over 49bp)
GGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAAC1423220.18631855330336963Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTC1421980.18615622070117446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1410820.18469522728141813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1410800.1846926090136408Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACA1379840.18063953049431677No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1372010.17961447865948776No Hit
GCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC1370360.1793984715678571Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACACGTCTGAACTC1365410.17875045029296519Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCACACGTCTGAACTCCA1349250.17663488992887358Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG1313120.17190498918910688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACAGATCGGAAGAGC1302100.17046232364379196No Hit
GGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACT1291110.16902358550014301Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGT1285870.16833759934247963No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG1220960.15984001127111913TruSeq Adapter, Index 5 (100% over 50bp)
GGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGT1179970.15447387146145855No Hit
GCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAACTC1170940.15329172355998905Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1160790.15196295266298845Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA1133070.1483340335235937No Hit
GCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAAC1108890.1451685477807883Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC1095720.1434444184494092Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT1090280.1427322496139724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA1059110.1386516792829863Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGGCCGGGCCACCCCTCCCAGATCGGAAGAGCACACGTCTGAAC1057690.13846578227079512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1057220.13840425297802758No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC1041090.136292620015602No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACA1039770.13611981434229747No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT996940.13051279389712153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC969810.12696111365715834No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC950090.12437950162870001No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCAGATCGGAAGAGCACA949400.12428917139038174No Hit
CCCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA947360.12402210807709296Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCAGATCGGAAGAGCAC936910.12265406316343225No Hit
GGCGGGTGTTGACGCGATGTGAGATCGGAAGAGCACACGTCTGAACTCCA930550.12182145401023778Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG925360.12114201352201774No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC922470.12076367382819198TruSeq Adapter, Index 5 (100% over 50bp)
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACACG905800.11858134763577816No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCAC904240.11837712274914555No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG900910.11794118116421827No Hit
TGGACGAGGCGCCGCCGCCCCAGATCGGAAGAGCACACGTCTGAACTCCA877290.11484900691917843Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAAC853430.11172541346081051Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT842970.11035605941326111Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT838870.10981931451890618Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGTCTG834590.1092590052145552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT829680.10861622047521795Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG823260.10777575651869145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC819860.10733065099654349No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA805550.10545728040185594No Hit
GGCGGGTGTTGACGCGATGTGAAGATCGGAAGAGCACACGTCTGAACTCC802210.10502002968303997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGTCTTGCCTAGGCAAGGCTAGATCGGAAGAGCACACGTCTGAACTCC799250.1046325260519935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGTC798540.10453957754589786No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG798400.10452124967145648No Hit
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGT796040.10421229407373024No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC794620.10402639706153902TruSeq Adapter, Index 5 (100% over 50bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG784420.10269108049509505No Hit
GGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGTCTGAACT774550.10139896534697723Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGT765240.10018016169662494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)