FastQCFastQC Report
Fri 7 Jul 2023
SRR7451196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73576551
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA14146601.922704966151512No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG13592891.8474486524925584TruSeq Adapter, Index 8 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT11002041.4953188006869198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA10265111.395160531512275No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC7864421.068875870520215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC6361940.8646695059136437Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT5775700.7849919466869274Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC5727090.7783852222157029Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA5692560.773692150913679No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC5275160.7169621201733144No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG4612850.6269456691439641No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4577960.6222036692097731No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4497350.6112477329903654No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC4484000.6094332962141702No Hit
AGTACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG4389390.5965745798549322Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG4007250.5446368368095972No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC3610780.49075146237827866No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3351600.4555255654753374Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA3310990.4500061439411587No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG3290750.44725526751043276No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3223760.4381504645413455No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG3056710.4154462200871579No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA3022360.4107776131012176No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA2876710.3909819040036275No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC2865300.3894311381896659No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG2861160.3888684589197447No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2733510.37151918143050766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2713980.3688648031354446No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2640320.35885346134259544No Hit
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC2513630.34163466020580385Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT2466240.3351937494324788No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2186640.29719251178272815No Hit
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC2171390.29511984055898466Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG2136430.29036832672409446Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2059160.2798663394809034No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG2013570.27367007186841363No Hit
CGGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACA1955060.2657178100125949No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC1917240.26057758537771086Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1865830.2535903048785203Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1849430.2513613338575764No Hit
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC1816760.24692106048841567Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGAACTCC1816270.2468544631835216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1750560.23792362868436168Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1682440.22866524417541673No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGATCGGAAGAGCACACGTCTGAAC1671880.22723000429851625Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1655490.22500239240624367No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1638150.22264566329019692Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCACACGTCTGAACTCCA1622780.22055668252239768Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1618420.21996410242170772No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC1546690.2102150724624208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA1522830.20697219145268173No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1498780.20370348699818777No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1486460.20202904047513723No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG1429420.19427657053400071No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC1280330.17401332117348095No Hit
CGCATCGACCTGGTATTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1256680.17079898186583928Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1255290.1706100629805276Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACA1166440.1585342047359627No Hit
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA1158440.1574469017989169Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1127020.1531765195136695Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1122050.15250103256402983No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1119530.15215853213886038No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGAGATCGGAAGA1116560.15175487092348214No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1116060.15168691448991675No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA1113050.1512778167598533No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC1091310.1483230710284313No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1076260.14627758237811392Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1045980.14216214076139558No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACA1032750.1403640135292561No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1022780.13900896224396275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA987970.13427783533914223No Hit
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC987290.13418541458949332Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA968590.13164384397414877Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC960580.13055518190843168No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG958180.13022899102731791No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA951180.12927760095740284No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG916920.12462122612950423No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC900530.12239361423723163No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA890810.12107254116872099Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA876980.11919286621630307Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT869700.1182034205435914No Hit
CGGCGGGTGCGGGGGTGGGCGGAGATCGGAAGAGCACACGTCTGAACTCC865430.1176230726009432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACA860470.11694894477997482No Hit
ACCCCTCCCACGGCGCGACAGATCGGAAGAGCACACGTCTGAACTCCAGT849380.11544167108349507Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGT840050.1141736040331654No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGAGATCGGAAGAGCACAC830700.11290281872549313No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC825570.11220558571711252No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG798820.10856991652136562No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT776640.10555536912840613No Hit
AGTACTTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG747420.10158399515084636Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCATCGACCTGGTATTGCAGTAGATCGGAAGAGCACACGTCTGAACTCC743690.10107704015644876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTTAGGTCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACA741130.10072910321659409No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT739730.10053882520261108No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACA738350.1003512654459707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)