FastQCFastQC Report
Fri 7 Jul 2023
SRR7451197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7451197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85268977
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1239583114.537328153942788TruSeq Adapter, Index 10 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT20913702.4526739660545007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC19768362.318353133285509Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC17154812.0118465828433707Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA14258531.6721826039967618No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA11223771.3162782520540852No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC7143970.8378158447942914No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG6953160.8154384214085271No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC6297650.738562865601167No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC5495830.6445286660352452No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA5410020.6344652170507452No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA5363580.629018922086986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT4332380.5080839658719021No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG4181880.49043393589675643No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC3823990.44846204733991357No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3770070.44213852829499756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3706530.4346868146430325Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG3653130.42842427909038944No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT3437160.4030961928861888No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA3290780.3859293398113595No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAG3232680.3791156073093266No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAG3225050.378220791836168No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC3050620.35776434845700095Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG2969170.34821222260002016No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACG2544640.29842506495650817No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC2490680.29209685487372505No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2429200.2848867296719181No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2334010.27372323230757184No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC2309120.2708042339947388No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACAC2269440.2661507244305277No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG2232850.2618595975415537No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2207220.2588538150281784TruSeq Adapter, Index 10 (100% over 50bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC2205730.2586790738676271No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC2203130.25837415640626255Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG2015080.23632041463333142TruSeq Adapter, Index 10 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2007460.23542677191963965TruSeq Adapter, Index 10 (100% over 50bp)
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC1919740.22513932587698338Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1909240.22390792843685692No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA1749550.205180132511734No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1742250.20432401810097944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC1739960.2040554561830852No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1721310.2018682597775273No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1693120.1985622508406545Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT1658120.19445759270689972No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC1629350.19108356372095328No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC1521070.17838492421458277No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1471060.17251995412118057Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1463770.17166501246989277No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA1426850.16733518451851487Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT1412050.16559950050766997No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG1401240.16433174752407315No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCAC1392800.16334193853410484No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT1357880.15924666247608435No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC1322550.1551033032799256Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1282650.15042399300744513Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1282330.15038646470450795Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1245530.14607070986673149Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT1224940.14365599812461688No Hit
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1145520.13434194243939387Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1144890.1342680585929863Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTACCTCCAGGAACGGTGCAGATCGGAAGAGCACACGTCTGAACTCCAG1117220.13102303314838643Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1093600.12825297528783533No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAAG1081980.12689022878742875TruSeq Adapter, Index 10 (97% over 49bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1061960.12454236433492101TruSeq Adapter, Index 10 (98% over 50bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC1048010.12290636487875303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT1027590.12051159004757381Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC980160.11494919189660269Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC976940.11457156334829723Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG976140.11447774259095427No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA935140.10966942877712724Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC914360.10723243460514367No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACA892810.1047051379542175No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGC889370.10430170869764276No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA871680.1022270972008964No Hit
CGGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACA853710.10011964843908003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)