FastQCFastQC Report
Sun 1 Oct 2023
SRR7471227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94213486
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length15-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA1054268011.190202642538882No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC60024956.371163253634411No Hit
ACGCGAAACTCAGGTGCTGCAATCT23769702.522961521665805No Hit
AAGAGGTGCACAATCGACCGATCCTGC22368822.3742694331467575No Hit
TTGCCTTGTTGTAGACGGCCTTGG19985202.1212674372329245No Hit
TCCAACGGGGCCTTTCCTTCTGGC18287831.9411053317780855No Hit
TCCACGTTCTAGCATTCAAGGTCCC17802631.8896052737078426No Hit
TCACGTTCTAGCATTCAAGGTCCC15964971.694552518733889No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC15960961.6941268896471997No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA15869471.684415965671836No Hit
ACGTTCTAGCATTCAAGGTCCC15228691.6164023481733816No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC13058671.3860722657051454No Hit
AGAGGTGCACAATCGACCGATCCTGA11443941.214681728261281No Hit
TTGCCTTGTTGTAGACGGCCTTGGT10259001.0889099252733307No Hit
AAGAGGTGCACAATCGACCGATCCTG9450521.0030963083140771No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA8834180.9376767992641732No Hit
ATATAGATGGATACGAATAAGGCGTCC7542410.8005658552959181No Hit
AAAGAGGTGCACAATCGACCGATCCTGA7150940.7590144790948506No Hit
TTTTCCACGTTCTAGCATTCAAGGTC6966730.7394620765863604No Hit
ACGTTCTAGCATTCAAGGTCCCA6108820.6484018646757217No Hit
AAATATAGATGGATACGAATAAGGCGTCC5585760.592883273632397No Hit
TCACGTTCTAGCATTCAAGGTCCCA5300600.5626158446148569No Hit
TCCACGTTCTAGCATTCAAGGTCCCA5195540.5514645748274297No Hit
ATATAGATGGATACGAATAAGGCGTC5154830.5471435373912392No Hit
ACTATGCCGACTAGGGATCGGGTGGTG4920540.5222755476853919No Hit
AAGAGGTGCACAATCGACCGATCCT3978210.4222548351517319No Hit
TCCACGTTCTAGCATTCAAGGTCC3807590.4041449012936428No Hit
AAATATAGATGGATACGAATAAGGCGTC3789050.4021770301546851No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA3773830.40056155017976935No Hit
CCAACGGGGCCTTTCCTTCTGGC3752270.39827313045183366No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCC3384050.35918955381822937No Hit
TTCCAACGGGGCCTTTCCTTCTGGC3328200.3532615277604737No Hit
TTTCCACGTTCTAGCATTCAAGGTCC3307060.3510176876376276No Hit
TACGCGAAACTCAGGTGCTGCAATCT3098360.3288658695847429No Hit
TAAGAGGTGCACAATCGACCGATCCTGA2896430.3074326323091367No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG2748820.2917650239584596No Hit
ACGTTCTAGCATTCAAGGTCC2693120.28585291918823597No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT2659610.2822961035535826No Hit
TTGGAAGAGATGTATTTATTAGATA2642820.28051398076916506No Hit
TCAATATAGATGGATACGAATAAGGCGTCC2608070.27682554915758023No Hit
TCACGTTCTAGCATTCAAGGTCC2606060.2766122039046512No Hit
ACGCGCAACTCAGGTGCTGCAATCT2378030.2524086625984734No Hit
CAAGAGGTGCACAATCGACCGATCCTGA2358400.25032509677011633No Hit
ATGATTAATAGGGACGGTCGGGGGC2330770.24739239560671814No Hit
AAGAGGTGCACAATCGACCGATCCTGAT2319970.24624606290441264No Hit
TATAGATGGATACGAATAAGGCGTCC2163660.22965501987687836No Hit
TTCACGTTCTAGCATTCAAGGTCCC1997820.21205244438147636No Hit
AAGAGGTGCACAATCGACCGATC1848460.196199087676259No Hit
AGAGATGTATTTATTAGATA1813180.19245440084872775No Hit
TCTATGCCGACTAGGGATCGGGTGGTG1748260.18556366760486923No Hit
TCAATATAGATGGATACGAATAAGGCGTC1735090.18416577855955782No Hit
TATAGATGGATACGAATAAGGCGTC1728040.18341747804555283No Hit
TCAAGAGGTGCACAATCGACCGATCCTGC1704930.18096453834645287No Hit
TTCCACGTTCTAGCATTCAAGGTCCC1672560.1775287244970428No Hit
TCAACGGGGCCTTTCCTTCTGGC1627400.1727353555307358No Hit
TAGAGATGTATTTATTAGATA1551330.16466113991366374No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC1465590.15556053196036077No Hit
TTTCCACGTTCTAGCATTCAAGGTC1461540.1551306571969962No Hit
TCCACGTTCTAGCATTCAAGGTC1451120.15402465842310517No Hit
ATGCCTTGTTGTAGACGGCCTTGG1386180.14713180234090903No Hit
ACACGTTCTAGCATTCAAGGTCCC1341340.14237239878800367No Hit
ATGTATTTATTAGATAAAAAATCA1326590.14080680551402164No Hit
TTGCCTTGTTGTAGACGGCCTTG1274740.1353033471237865No Hit
TCCAACGGGGCCTTTCCTTCTGG1259390.13367406869967638No Hit
ATGTATTTATTAGATAAAAAATCAATG1239460.13155866029625526No Hit
AGAGGTGCACAATCGACCGCTCCTGA1234980.13108314450863223No Hit
AGAGGTGCACAATCGACCGATCCT1209870.12841792097577198No Hit
TTGTGGCGTCGCTGAACCATAGC1189410.12624625735640438No Hit
TCGGGTCTTGTAATTGGAATGAGT1181690.1254268417580897No Hit
TTTCGGTGCCCGAGTTGTAATTTG1127920.11971959088744472No Hit
TTTCCCCGTTCTAGCATTCAAGGTCCC1118350.11870381274290179No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA1101770.11694397976102912No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCT1059540.11246160661118092No Hit
TTCGGTGCCCGAGTTGTAATTTG1048530.1112929841063306No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCC1026140.10891646658738431No Hit
TAGAGGTGCACAATCGACCGATCCTGA1022760.10855770690832946No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG1016660.10791024121536061No Hit
TCCAACGGGGCCTTTCCTTCTGGCT988300.10490005645263992No Hit
TTTCCACGTTCTAGCATTCCAGGTCCC984310.10447655020428817No Hit
ATTTCCACGTTCTAGCATTCAAGGTCCC974940.10348200044312127No Hit
TCACGTTCTAGCATTCAAGGTC960930.10199495218763055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)