FastQCFastQC Report
Sun 1 Oct 2023
SRR7471228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23139976
Total Bases684 Mbp
Sequences flagged as poor quality0
Sequence length15-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCC3252461.4055589340282808No Hit
AAGAGGTGCACAATCGACCGATCCTGA3140281.3570800592014443No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC2645791.1433849369593123No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA2452021.0596467342922051No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC2255500.9747201120692606No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA2145150.9270320764377629No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC1946030.8409818575438454No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1666830.7203248611839529No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1508310.6518200364598477No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC1501210.6487517532429593No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG1483060.6409081841744347No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC1374260.5938899850198635No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1319690.5703074195063988No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG1294440.5593955672209859No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA1176850.5085787470133937No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT1153250.49837994646148287No Hit
AAAAAAAAAAAAAAA983380.42497019011601395No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG894970.3867635817772672No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC888880.3841317726517953No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGC869580.37579122813264804No Hit
TCCAACGGGGCCTTTCCTTCTGGC818700.3538033055868338No Hit
GCGTAGTCGGTAGCGCTCTCCCT799870.3456658727735932No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC760280.3285569526951973No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGC735440.31782228296174553No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCC727370.314334811756071No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGC726690.3140409480113549No Hit
TCCACGTTCTAGCATTCAAGGTCCC693730.2997971994439407No Hit
GAAGCGGTGGCTCAATGGTAGAGCTTTCGACT673260.2909510364228554No Hit
AAGAGGTGCACAATCGACCGATCCTGC666690.28811179406581927No Hit
TGGGCGGTTAGTGTAGTGGTTATCATCCCACC663510.2867375489067059No Hit
TACGCGAAACTCAGGTGCTGCAATCT652350.2819147262728362No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT633610.2738161871905139No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGC625010.27009967512498717No Hit
TTTGTGCCCTCTGCTCCTTGTGGGTAGGGGA623890.2696156642513372No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA623330.26937365881451214No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCT612000.2644773702444635No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC593740.2565862643937055No Hit
GTCTAGTCGGTTATGGCATCTGCT573370.24778331662919617No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCG571660.2470443357417484No Hit
TTGCCTTGTTGTAGACGGCCTTGG537080.2321005000178047No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT534630.23104172623169533No Hit
ACGCGAAACTCAGGTGCTGCAATCT527270.22786108334770963No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG521630.22542374287682926No Hit
TTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGA516680.22328458767632256No Hit
GTCTAGTGGTATGATTCTCGCT512480.2214695469001351No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCC503320.2175110293977833No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG492920.21301664271389048No Hit
AGAGGTGCACAATCGACCGATCCTGA492320.21275735117443512No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA480900.20782216887346816No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCC467070.20184549888902217No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG452320.195471248544078No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT448830.19396303608957935No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGG438090.18932171753332847No Hit
TCCGTGATAGTTTAATGGTCAGACTGGGC437450.18904513989124277No Hit
ATGTACACGGAGTCGACCCGCAACGCGA436880.18879881292876016No Hit
TTGCCTTGTTGTAGACGGCCTTGGT431660.18654297653549856No Hit
TTCCGTGATAGTTTAATGGTCAGCATGGGC430310.18595957057172402No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGC422110.1824159195325008No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC419180.18114971251482714No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCC414560.1791531676610209No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT390250.1686475387874214No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCC370720.16020759917814953No Hit
TCCGATATAGTGTAACGGCTATCACATCCCGC348970.15080828087289286No Hit
TCCACGTTCTAGCATTCAAGGTCCCA339090.14653861352319467No Hit
TCCAACGGGGCCTTTCCTTCTGGCT337720.14594656450810495No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA324340.14016436317825048No Hit
TCCGATATAGTGTAACGGCTATCCCATCACGC322870.13952909890658488No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA316210.1366509628186304No Hit
AAGCGGTGGCTCAATGGTAGAGCTTTCGACT315500.13634413449694155No Hit
TTTTCCACGTTCTAGCATTCAAGGTC311250.13450748609246613No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG311040.13441673405365676No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTC301850.1304452519743322No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGT300470.1298488814335849No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGA297630.12862156814682954No Hit
TCCGTGATAGTTTAATGGTCAGACTGGGCG294740.12737264723178623No Hit
TGGGCGTGTGGTCTAGTGGTATGATTCTCGCT292980.12661205871605052No Hit
AAGAGGTGCACAATCGACCGATCCTG291990.1261842276759492No Hit
TCACGTTCTAGCATTCAAGGTCCC285000.12316348124129428No Hit
AGAGATGTATTTATTAGATAAAAAATCA284320.12286961749657821No Hit
TATACGCGAAACTCAGGTGCTGCAATCT280830.12136140504207955No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCC276940.11968033156127733No Hit
AAAAAAAAAAAAAAAA271270.11723002651342421No Hit
GTCTAGTCGGTTATGGCATCTGC267970.11580392304641975No Hit
TCCGATATAGTGTAACGGCTATCACATCCCG263120.11370798310248896No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG250560.10828014687655682No Hit
TTCCAACGGGGCCTTTCCTTCTGGC249020.10761463192528808No Hit
TTACGCGAAACTCAGGTGCTGCAATCT243850.10538040316031444No Hit
GCTCAATGGTAGAGCTTTCGACT243190.10509518246691353No Hit
TCCGATATAGTGTAACGGCTATCCCATCACG237270.10253683927762068No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC235290.10168117719741801No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT235100.10159906820992383No Hit
AGGTGGGGACACGGGTTCGATTCTCGTACCGCTC234050.10114530801587694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)