FastQCFastQC Report
Sun 1 Oct 2023
SRR7471229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34404121
Total Bases972.7 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA1252725636.41207982032152No Hit
ATGTATTTATTAGATAAAAAATCAATG27698858.051026794144805No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT5849261.700162605520426No Hit
ACGCGAAACTCAGGTGCTGCAATCT5450521.5842636991074415No Hit
AGAGATGTATTTATTAGATAAAAAATCA4031201.1717200971360378No Hit
AAGAGGTGCACAATCGACCGATCCTGA3854921.120482049228928No Hit
TGATGTATTTATTAGATAAAAAATCAATG3176400.9232614895174912No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA3036480.8825919429826445No Hit
ACGTTCTAGCATTCAAGGTCCCA2899810.8428670507233713No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG2584350.7511745467933915No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA2408840.7001603092838791No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA2221790.6457918224389456No Hit
GATGTATTTATTAGATAAAAAATCAATG1877760.5457950807695392No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1685500.4899122404551478No Hit
ACACGGAGTCGACCCGCAACGCGA1665770.4841774623452813No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC1549050.4502512940237595No Hit
TACGCGAAACTCAGGTGCTGCAATCT1517340.44103437492270187No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC1407140.40900332840940773No Hit
AGAGATGTATTTATTAGATAAAAAATC1389550.40389056880714963No Hit
ACGTTCTAGCATTCAAGGTCCC1265540.3678454682798029No Hit
ATTTATTAGATAAAAAATCAATG1252070.363930239636118No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1224960.3560503696635644No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1157680.3364945728449217No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT1137620.33066387599322766No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT1135020.3299081525727688No Hit
ACTATGCCGACTAGGGATCGGGTGGTG884880.25720174626754744No Hit
ATGTACACGGAGTCGACCCGCAACGCG882080.25638789027628406No Hit
ACGGAGTCGACCCGCAACGCGA869250.25265868585917367No Hit
ATTACCCAATCCTAATTCAGGGAGGTAG657630.1911486126909041No Hit
ATGTATTTATTAGATAAAAAATCAAT655750.19060216652534154No Hit
TCCGATATAGTGTAACGGCTATCACATCACG639590.1859050548043358No Hit
ATGTACACGGAGTCGACCCGCAACGC627810.18248104638394919No Hit
TACACGGAGTCGACCCGCAACGCGA623910.1813474612532609No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC622630.18097541280011195No Hit
CAAGAGGTGCACAATCGACCGATCCTGA535150.155548226330212No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG506890.14733409407553238No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT424600.12341544781800994No Hit
ATACGCGAAACTCAGGTGCTGCAATCT412830.11999434602616355No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT410380.1192822220338081No Hit
AGGTACACGGAGTCGACCCGCAACGCGA409580.11904969175058999No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCG403470.1172737417125117No Hit
TGTACACGGAGTCGACCCGCAACGCGA387630.11266964210479319No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG387250.11255919022026459No Hit
ATGTACACGGAGTCGACCCGCAACGAGA384150.11165813537279444No Hit
TTGGAAGAGATGTATTTATTAGATAA360990.10492638367363026No Hit
GTCTAGTCGGTTATGGCATCTGCT354220.10295859615189704No Hit
GTCTAGTCGGTTATGGCATCTGC352870.10256620129896647No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCT346530.10072339880446297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)