FastQCFastQC Report
Sun 1 Oct 2023
SRR7471230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26921095
Total Bases761.7 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA1775057165.93554608384244No Hit
ACACGGAGTCGACCCGCAACGCGA2432090.9034142184781115No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC2300090.8543820375805665No Hit
ACGGAGTCGACCCGCAACGCGA1912730.7104948740012247No Hit
ATGTACACGGAGTCGACCCGCAACGCG1471500.546597380232862No Hit
ATGTACACGGAGTCGACCCGCAACGC1167790.4337825040177601No Hit
TACACGGAGTCGACCCGCAACGCGA1155170.42909473035922197No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1140920.42380148355778247No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG993220.3689374447807565No Hit
TCCGATATAGTGTAACGGCTATCACATCACG672480.24979667431803942No Hit
TGTACACGGAGTCGACCCGCAACGCGA623140.23146903942800248No Hit
ATGTATTTATTAGATAAAAAATCAATG591690.21978675087324642No Hit
AGGTACACGGAGTCGACCCGCAACGCGA587500.21823035058566526No Hit
ACGCGAAACTCAGGTGCTGCAATCT498490.18516705951225237No Hit
ATGTACACGGAGTCGACCCGCAACGAGA497350.1847435997681372No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAG492940.18310547917906014No Hit
TATGTACACGGAGTCGACCCGCAACGCGA475470.1766161443284532No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGT453780.16855926551278838No Hit
ATGTACACGGAGTCGACCCACAACGCGA448650.16665369666427016No Hit
ATGTACACGGGAGTCGACCCGCAACGCGA424160.15755674128411196No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA417650.15513856327166484No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC384820.1429436655529799No Hit
AATGTACACGGAGTCGACCCGCAACGCGA382240.14198530928998246No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT381090.14155813498670838No Hit
GTCTAGTGGTATGATTCTCGCT371270.13791043789266372No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGC365950.1359342924201263No Hit
ATGTACACGGAGTCGACCCGCAACGCGGA353340.13125023332074717No Hit
ACGTTCTAGCATTCAAGGTCCCA349730.1299092774643825No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT341390.12681133512585577No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGC313700.11652572081484798No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA312880.11622112696381777No Hit
ATGTACACGGAGTCGACCCGCAACGGCGA307820.11434156002941187No Hit
ATATATGATGATATCCTATAACAACAACA300980.1118008015647209No Hit
AGAGATGTATTTATTAGATAAAAAATCA286220.10631811224617721No Hit
NTGTACACGGAGTCGACCCGCAACGCGA282080.10478028475439055No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG279170.10369934803914921No Hit
ATGTNCACGGAGTCGACCCGCAACGCGA278280.10336875227400669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)