FastQCFastQC Report
Sun 1 Oct 2023
SRR7471231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38469682
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length15-45
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCC26407046.864376991730786No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA22498455.848358715312489No Hit
TCCAACGGGGCCTTTCCTTCTGGC12417563.2278821540557576No Hit
AAGAGGTGCACAATCGACCGATCCTGA6554971.7039314231919047No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC5946691.5458121021119957No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA5338481.3877109771793799No Hit
TTGCCTTGTTGTAGACGGCCTTGG4931471.2819107784670536No Hit
TTGGAAGAGATGTATTTATTAGATA4094651.0643836359239984No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC3739240.9719965972164781No Hit
TTCCAACGGGGCCTTTCCTTCTGGC3704990.9630934822908076No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC3432000.89213110729639No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA3032340.7882415040498645No Hit
TTGCCTTGTTGTAGACGGCCTTGGT2910040.756450235278784No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA2628050.6831483556323652No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG2585910.6721942749617738No Hit
ACGCGAAACTCAGGTGCTGCAATCT2230280.57975004836276No Hit
TACGCGAAACTCAGGTGCTGCAATCT1884210.48979089559409406No Hit
TTTTCCACGTTCTAGCATTCAAGGTC1662710.4322130866587356No Hit
CCAACGGGGCCTTTCCTTCTGGC1636710.425454517664066No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG1624430.4222623935388912No Hit
AGAGATGTATTTATTAGATAAAAAATCA1611310.4188519156461964No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1466650.3812482775397No Hit
TTTCCAACGGGGCCTTTCCTTCTGGC1366960.35533436434436866No Hit
TCCAACGGGGCCTTTCCTTCTGGCT1361960.35403463953770137No Hit
TGATGTATTTATTAGATAAAAAATCA1278950.33245660829741197No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG1222620.3178139086254989No Hit
TCCACGTTCTAGCATTCAAGGTCCC1195990.3108915743051892No Hit
TGATGTATTTATTAGATAAAAAATCAATG1080030.28074835658896274No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG1037700.2697448863757179No Hit
TTTGGAAGAGATGTATTTATTAGATA1030280.26781609476262375No Hit
AAGAGGTGCACAATCGACCGATCCTGC1012060.2630798975671283No Hit
ATGTATTTATTAGATAAAAAATCAATG1009670.2624586291095414No Hit
TCCACGTTCTAGCATTCAAGGTCCCA927990.24122632466782543No Hit
AGAGATGTATTTATTAGATAAAAAATC923330.24001498114801156No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT907650.2359390441543031No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT897790.23337598683555533No Hit
TTACGCGAAACTCAGGTGCTGCAATCT869280.22596495598793875No Hit
ACTATGCCGACTAGGGATCGGGTGGTG864140.22462883888668483No Hit
ATGTATTTATTAGATAAAAAATCA841610.2187722789078423No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGG821440.21352918903774668No Hit
AGAGGTGCACAATCGACCGATCCTGA813150.21137424530829235No Hit
TCAATATAGATGGATACGAATAAGGCGTCC797070.20719433033005055No Hit
TTTCCACGTTCTAGCATTCAAGGTCC793670.20631051746151682No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCC774890.20142875108767472No Hit
TCCAACGGGGCCTTTCCTTCTGG764870.19882410257511357No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT698100.1814675775068793No Hit
TTGACGTGGGTTAGTCGATCCTAAGAGATGGG688370.17893831303310487No Hit
AACTATGCCGACTAGGGATCGGGTGGTG688270.17891231853697154No Hit
AAATATAGATGGATACGAATAAGGCGTCC684590.17795572107926444No Hit
TCCAGGGGGCATGCCTGTTTGAGC676600.1758787608382102No Hit
TGATGTATTTATTAGATAAAAAATC676050.17573579110947682No Hit
TGGAAGAGATGTATTTATTAGATA668860.17386678683748932No Hit
ACGTTCTAGCATTCAAGGTCCCA664420.17271263120916883No Hit
AAGAGGTGCACAATCGACCGATCCTGAT641280.16669750480391288No Hit
TTGGAAGAGATGTATTTATTAGAT622080.16170656154631066No Hit
TAGAGATGTATTTATTAGATAAAAAATCA597920.1554262912804946No Hit
TCACGTTCTAGCATTCAAGGTCCC569370.1480048626344247No Hit
CAAGAGGTGCACAATCGACCGATCCTGA549090.14273317881858238No Hit
TCACGTTCTAGCATTCAAGGTCCCA541340.14071860536824818No Hit
ACGTTCTAGCATTCAAGGTCCC512030.13309961855156485No Hit
TCGTGGGTTAGTCGATCCTAAGAGATGGG505210.13132679391527075No Hit
TTGATGTTGACGCAATGTGATTTCTGCC498220.12950977863554994No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT498130.12948638358902992No Hit
TGATGTTGACGCAATGTGATTTCTGCCCA496040.12894309861984302No Hit
TCCGGAGATGGGGTCTTATGGCTGG491580.12778374409229584No Hit
ATATAGATGGATACGAATAAGGCGTCC483840.12577177009157497No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA481680.12521028897509473No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT469360.12200776705146665No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG469320.12199736925301331No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC464100.12064045655485271No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGG452790.11770047904217143No Hit
AAAGAGGTGCACAATCGACCGATCCTGA452440.11760949830570473No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGG429430.11162816474542213No Hit
AAGAGGTGCACAATCGACCGATCCT424560.11036223278372824No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT421950.10968377643464794No Hit
TTGGAAGAGATGTATTTATTAGATAA420110.10920547770579439No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGC419870.10914309091507436No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC418400.10876097182191419No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCA415700.10805912042631391No Hit
AAAAAAAAAAAAAAA414110.10764580793779371No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT413210.10741185747259362No Hit
TATACGCGAAACTCAGGTGCTGCAATCT412530.10723509489888687No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC406400.10564163228591285No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCC395440.10279263550969826No Hit
CCGGAGATGGGGTCTTATGGCTGG390380.10147731400535101No Hit
TGGACGTGGGTTAGTCGATCCTAAGAGATGGG385840.10029716388089717No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCC384930.10006061396608373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)