FastQCFastQC Report
Sun 1 Oct 2023
SRR7471234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18884840
Total Bases482.8 Mbp
Sequences flagged as poor quality0
Sequence length20-45
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCGA12907066.834614431469898No Hit
ACGCGAAACTCAGGTGCTGCAATCT2548861.349685779704779No Hit
ACGGAGTCGACCCGCAACGCGA1574530.8337534233808706No Hit
ATCGGGCGTTCGACTCGCCCCCGGGAGA1553620.8226810499850674No Hit
ATGTATTTATTAGATAAAAAATCAATG1165910.6173788075514539No Hit
ATCTGGTTGATCCTGCCAGTAGTC1054160.5582043586284025No Hit
ACGTTCTAGCATTCAAGGTCCCA993130.5258874314000013No Hit
ACACGGAGTCGACCCGCAACGCGA971310.5143331900084935No Hit
AGAGATGTATTTATTAGATAAAAAATCA887340.4698689530861792No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA842970.44637391685606015No Hit
AAGCCTTTGTTGTCTGATTTGT802850.4251293630234622No Hit
ACGTTCTAGCATTCAAGGTCCC713600.37786923267552175No Hit
ATTTATTAGATAAAAAATCAATG712180.37711730679211475No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACC705140.37338944889127995No Hit
TACGCGAAACTCAGGTGCTGCAATCT648810.343561290431902No Hit
ATGTATTTATTAGATAAAAAATCA619030.327792027891155No Hit
AAACATAGCATTGCGATGGTCA534120.28283003721503597No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA516360.27342566841974836No Hit
TGCAAGAGTTCGAATCTCTTAGCAACC511840.27103221419932605No Hit
ACCTACTAAATAGTGGTGCTAGCA484310.2564543835160902No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCAC476520.2523293816627517No Hit
TGATGTATTTATTAGATAAAAAATCAATG474810.25142389345104327No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA467470.24753717796920705No Hit
TCCAACGGGGCCTTTCCTTCTGGC442090.23409782661648182No Hit
AAGAGGTGCACAATCGACCGATCCTGA427130.22617612857720798No Hit
ATTTATTAGATAAAAAATCA408650.21639050158751677No Hit
TCCAGGGGGCATGCCTGTTTGAGC408500.216311072797016No Hit
ATGCAAGAGTTCGAATCTCTTAGCAACC400620.21213841366937713No Hit
AAGATGGTGAACTATGCCTGAAT371440.19668686629063312No Hit
ACGGGCTTGGCAGAATCAGCGGGGA370010.19592964515452604No Hit
AAATTCTAGAGCTAATACATGCTT364970.19326083779370118No Hit
AAAACATAGCATTGCGATGGTCA364320.192916646368198No Hit
AGAATAGGACGTTTGGTTCTAT332050.17582886590513871No Hit
ATCGGGCGTTCGGCTCGCCCCCGGGAGA298170.15788854975737152No Hit
AGAGATGTATTTATTAGATAAAAAATC296040.15676066093226101No Hit
AATTCTAGAGCTAATACATGCTT286580.15175135187801433No Hit
ATGCAAGAGTTCGAATCTCTTAGCAACCAC284240.15051226274620277No Hit
TGATGTATTTATTAGATAAAAAATCA280430.14849477146748397No Hit
TCCAACGGGGCCTTTCCTTCTGGCT278180.14730333960997286No Hit
TAGTCTTTCGCTCCTATCTGAAC268830.14235227833542674No Hit
ATGTACACGGAGTCGACCCGCAACGC266970.14136736133321753No Hit
TCTTTGGGCATTCGAGCAATCGGGGCCCAGAGG265410.14054130191200984No Hit
ACTTATGTTGCCTTCCTTCTGAAA265210.14043539685800888No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACC260290.13783013252958456No Hit
ACGTCGGCGCGAGCCCTGGGAGGAG258750.13701466361377695No Hit
TACACGGAGTCGACCCGCAACGCGA255370.13522486820116028No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGT252270.133583339864145No Hit
ATATGCTTGTCTCAAAGATTAAGCC247140.13086687522901969No Hit
ATATATGATGATATCCTATAACAACA244320.12937361396760577No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGA242240.12827220140599552No Hit
TTGTGCCCTCTGCTCCTTGTGGGTAGGGGA230530.12207146049423771No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT226960.12018105528032008No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA222080.117596971962696No Hit
ATGCTTGTCTCAAAGATTAAGCC221300.11718394225209215No Hit
TTGTCCGCGCGAGTTCGAACCTCGCATCCT220980.11701449416569058No Hit
ATCTGGTTGATCCTGCCAGT218950.11593955786758055No Hit
ATGCAAGAGTTCGAATCTCTTAGCAACCA218240.11556359492587705No Hit
ATGCATGTCTAAGTATAAGCAA217970.11542062310297573No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC213780.11320191222165504No Hit
ACTATTCAGTTCTGCTTCTGAAC212150.11233878603154698No Hit
TATCTGGTTGATCCTGCCAGTAGTC212080.11230171926264665No Hit
AAACATAGCATTGCGATGGTCAG209830.11111028740513555No Hit
ATGTACACGGAGTCGACCCGC206730.10946875906812026No Hit
TAAGCCTTTGTTGTCTGATTTGT203880.10795961204860618No Hit
TACTATTCAGTTCTGCTTCTGAAC200730.10629160744809063No Hit
ATCGGGCGTTCGACTCGCCCCCGGGAGAC199750.10577267268348581No Hit
ATGCCGACTAGGGATCGGGTGGTGTTT198770.10525373791888096No Hit
AAGTCTGGTGCCAGCAGCCGCGGTA198030.10486188921907731No Hit
TCGGGCTTGGCAGAATCAGCGGGGA190280.10075806837653906No Hit
AACAAACACTGAAAATTCTGAAA190180.10070511584953858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)