FastQCFastQC Report
Sun 1 Oct 2023
SRR7471235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54742659
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT18558883.390204337717684No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT13228772.4165377133032577No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT12153362.2200894552820314No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC7007871.280147900744098No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA6658061.2162470953411306No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG6483091.1842848189014714No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA3623620.6619371558111564No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT2935580.5362508971294215No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT2860660.5225650438353753No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG2534720.4630246404362638No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT2466030.4504768392781213No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA2238010.40882376575825446No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT2209870.40368335049271176No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT1990380.36358847676726846No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG1982190.3620923857571478No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1699480.31044893160925924No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA1689950.30870805891982706No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1611750.2944230385301525Illumina Single End Adapter 1 (95% over 22bp)
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG1535230.28044490860409244No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC1493160.2727598599110796No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC1453430.2655022657923869No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC1424000.260126202492283No Hit
ATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATTCGTATG1404110.2564928386105615No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG1341210.24500271351451888No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1323570.24178036364656674No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG1319950.24111908776663551No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT1219980.2228572784526232No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG1215320.22200602276188303No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG1215000.22194756743548028No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT1175950.2148141908853934No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATTCGTAT1096570.20031361647960869No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1074260.19623818419196626No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATTCGTATG1062510.19409177767561492No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATTCG1009010.18431877779265343No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT999050.1824993557583675No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGT996500.18203353987609555No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA970630.1773077920822224No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT942140.17210344130342664No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG925360.1690381901251819No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATTCGT911570.16651913090301296No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATTCG892210.16298258365564597No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG812510.1484235539234585No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT805820.14720147225585078No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT766910.14009366991106514No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT742980.13572230753350875No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT730500.1334425498038011No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC713640.13036268479395566No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC712430.13014165059099522No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGGCTGTAGGCACCATCAATTCG708130.12935615714245813No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG704150.1286291190203238No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG696110.12716042894445445No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC695140.1269832362362961No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG646480.11809437316517635No Hit
ACGGGCTTGGCAGAATCAGCGGGGACTGTAGGCACCATCAATTCGTATGC621740.11357504574266296No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT606930.1108696601675852No Hit
ACAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG590780.1079194929131959No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT587210.10726735067801511No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT568690.10388424866245537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)