FastQCFastQC Report
Sun 1 Oct 2023
SRR7471236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62637379
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1143404018.25433979285755Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA41233006.582810561086855Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA26891344.293177720606732No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT7931541.266263072725313No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7575511.2094232103804983Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT6170360.9850923040697472No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4123560.6583225648697721Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG3947020.6301381160919904Illumina Single End Adapter 1 (95% over 22bp)
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT3800260.606708016949432No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA3220590.514164234106922No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT3066490.4895623107090736Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA2833880.45242633795389176No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2687410.42904253704485307Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA2630740.41999522361879155Illumina Single End Adapter 1 (95% over 22bp)
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC2256790.3602944497406253No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC2240070.3576251171045966No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2172310.34680729536911176Illumina Single End Adapter 1 (95% over 22bp)
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2032260.3244484415607492No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1956720.31238854997428933Illumina Single End Adapter 1 (95% over 22bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1954250.3119942167439669No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA1654660.26416494853656No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1600270.2554816350154115No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1463070.2335777810881902No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT1312590.20955378736393168No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATC1185650.1892879330088189No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTTCTGTAGGCACCATCAA1178400.18813047717082798No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC1158160.18489917976931952No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG1090830.17415000713870865Illumina Single End Adapter 1 (95% over 22bp)
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA1039810.1660047110208746No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1016100.16221943130794156Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA1008360.16098374742021054Illumina Single End Adapter 1 (95% over 22bp)
ATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCTGTAGGCACCATCAAT995350.15890671287507097No Hit
AACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA963310.1537915563165566Illumina Single End Adapter 1 (95% over 22bp)
CTGTATGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA931250.1486732067764202Illumina PCR Primer Index 1 (95% over 22bp)
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG921010.1470384001859337No Hit
TCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA913440.14582985664199008Illumina Single End Adapter 1 (95% over 22bp)
ACAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG899610.14362190985034673No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG848760.13550375407630003No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT812130.12965580823552658No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT770910.12307507311249406No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG758970.12116886308413384No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA712300.11371804046909433No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG711760.11363182996529915No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT692810.11060647987841253No Hit
ATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATTCGTATG684430.10926862057877612No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC677780.10820695418944652No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC676170.10794991916887198No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCCTGTAGGCACCATCAA675800.10789084900886417No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG660520.10545141104962262No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA658670.10515606024958356No Hit
CTGTAGGCACATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA658460.1051225339425521Illumina Single End Adapter 1 (95% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA656530.10481441121602485No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA638750.10197585055402782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)