FastQCFastQC Report
Sun 1 Oct 2023
SRR7471238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30595179
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT9802943.204079963055617No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT9438413.084933740704704No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT7916222.5874076435375652No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC5913231.9327326047021982No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC5174311.6912174300402034No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC4879981.5950159990892683No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA3803211.243074930203873No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG3681401.203261468089466No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA2890160.9446455600079999No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1633110.5337801749746258No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG1497640.48950195715475303No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT1402520.45841209165666263No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1381030.4513881092181223No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG1325210.4331434047174556No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT1188750.38854160650604463No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1180090.3857110952022866No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA1165870.38106330412382944No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT1164620.38065474302340246No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG1137930.3719311464070859No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT1084370.3544251203759913No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG1013150.3311469431180645No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG1012620.33097371321148344No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG943150.3082675214941544No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTATG902320.29492228170980794No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT854780.27938388593836955No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT842570.275393061109399No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA781920.2555696765166826No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATTCGTATG778020.25429496588335043No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATTCGTAT775210.25337652052959064No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA768710.2512520028073704No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT746270.2439175139325055No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATTCGTATGC733720.23981556048421876No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC725620.237168084553452No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT714610.23356947838089134No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA708770.23166068091969652Illumina Single End Adapter 1 (95% over 22bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA705430.23056900565935567No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG704010.23010488024927064No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT587980.19218060466323794No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT563610.1842152974493138No Hit
ATGTATTTATTAGATAAAAAATCAACTGTAGGCACCATCAATTCGTATGC549720.17967536650136937No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC544680.17802804814444786No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG530020.1732364435586404No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC525920.17189636314923995No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGTA521760.170536671807019No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGT498550.1629505092942911No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG488100.15953493849472167No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATTCGT468990.1532888563913942No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATTCG421220.13767528537747728No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT400950.1310500585729536No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATTCG378470.12370249574287503No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG364470.11912661141809303No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGCCGTC363580.11883571591458902No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC359680.11756100528125689No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC354520.11587446505869439No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG353770.11562932839843819No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT353200.1154430245366435No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG337040.11016114663032368No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC335000.10949437491442687No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC332160.10856612409425681No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC310800.10158463201016081No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG306720.1002510885783672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)