FastQCFastQC Report
Sun 1 Oct 2023
SRR7471239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20645452
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9023584.37073501708754Illumina Single End Adapter 1 (95% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT6757243.2729920371808765No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT6400193.100048378693767No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAA3406151.6498306745718136No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC3125721.5139993059972723No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAA2573961.2467443192815542No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC2555721.237909443687646No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC2463061.1930278881760497No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1817040.8801163568615499No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAAT1573020.7619208337022604No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC1476520.7151793043814202No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1364010.6606830405069358No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAAT1301020.6301726888808247No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1256380.6085504933483655Illumina Single End Adapter 1 (95% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATT1223470.5926099365613308No Hit
TCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCCTGTAGGCACCATCAA1053460.5102625023661386No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT1030350.49906875373811144No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT954620.4623875515052904No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG906260.43896350634512626No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGCTGTAGGCACCATCAATT786470.3809410421239506No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG768780.37237256902876237No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATTCG684500.3315500188612969No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGCTTCGGACTGTAGGCACCATCAA644630.3122382595450078No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA593610.2875257950273988No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGGCTGTAGGCACCATCAATTCG532400.2578776187607808No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA504460.24434437182581423No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAATTCG496070.24028052279988832No Hit
CCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGACTGTAGGCACCATCAAT486330.23556277673165016No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC472190.22871381067365343No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT436290.2113249930299419No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCTGTAGGCACCATCAATTCGTA435990.21117968257609473No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA433900.21016735308095943No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG430300.20842362763479338No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATT420560.2037058815665552No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATT417590.2022673080734682No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG403610.19549584092419No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG402060.1947450702459796No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA393410.19055528549338613No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA377260.1827327393946134Illumina Single End Adapter 1 (95% over 22bp)
TCCGATGTAGTGTAACGGCTATCACATCACGTCTGTAGGCACCATCAATT368460.17847029941509635No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC364290.17645048410662068No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA363000.17582564915507784No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG361080.1748956622504559No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGCTGTAGGCACCATCAAT360310.17452269875224818No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA352630.1708027511337606No Hit
ATGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT350520.16978073427503548No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGCCTGTAGGCACCATCAA345490.1673443623321979No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATTCGTA344980.1670973345606577No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATTCG343340.1663029707462932No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATTCGTATGCCGT336610.16304317289832163No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTCG330090.1598850923680431No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT328160.15895026178162627No Hit
ATGCGAAAGCAGTTGAAGACAAGTTCTGTAGGCACCATCAATTCGTATGC327580.15866932823752175No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT324140.1570031017000742No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAA321700.15582124334211717No Hit
CGTGGAGACCGGGGTTCGACTCCCCGCTTCGGACTGTAGGCACCATCAAT317570.1538208027608211No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT313830.1520092657695264No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA311910.1510792788649045No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG300340.14547513902819856No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCTGTAGGCACCATCAAT298210.14444343480588365No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGCTGTAGGCACCATCAAT296100.1434214179471585No Hit
TTGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC295070.14292251872228323No Hit
TCGGGGATCAGCGGTTCGTTCCCGCTAGAGACTGTAGGCACCATCAATTC286710.13887320074174206No Hit
TGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAAT286110.1385825798340477No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATT285860.13846148778917508No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT283420.13727962943121808Illumina Single End Adapter 1 (95% over 22bp)
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA277880.1345962297168403No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC274120.13277500536195574Illumina Single End Adapter 1 (95% over 22bp)
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA273850.1326442259534933Illumina Single End Adapter 1 (95% over 22bp)
ATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCCTGTAGGCACCATCAATT272180.131835331093744No Hit
AGGAGAAGACTGCGGGTTCGAGTCCCGTACGGACTGTAGGCACCATCAAT270620.13107971673373875No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC269510.1305420680545042No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC269340.13045972546399082No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG266210.12894365306218533No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC260510.12618275443908905No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG259770.12582432198626603No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCTGTAGGCACCATCA258660.1252866733070315No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT253060.12257421150188429No Hit
TATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATT251800.12196390759572617No Hit
TGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAATTC248280.12025893160391934No Hit
TCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTGTAGGCACCATCAAT247860.1200554969685333No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCCTGTAGGCACCATCAATT246880.11958081615263255No Hit
TGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTCG236640.11462088599464909No Hit
TCGTGGTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGT231890.11232013714206886No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACTGTAGGCACCATCA226710.1098111099723077No Hit
ATGGAATAATAGAATAGGACGTTTGGTTCTACTGTAGGCACCATCAATTC220970.10703083662203182No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT220110.10661427998766994No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG209020.10124263687711947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)