FastQCFastQC Report
Sun 1 Oct 2023
SRR7471240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20600542
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8264254.0116662949935975Illumina Single End Adapter 1 (95% over 22bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT6686353.2457155738912116No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT6665653.2356672945789486No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAA3377551.6395442411175396No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC3089911.49991684684801No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAA2548551.237127644505664No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC2523241.2248415599939069No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC2393431.161828654799471No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1791510.8696421676672391No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAAT1560010.7572664835711604No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC1471740.7144180963782409No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1409930.6844140314366486No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAAT1247960.6057898864991027No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1208790.5867758236652221Illumina Single End Adapter 1 (95% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATT1206620.5857224533218592No Hit
TCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCCTGTAGGCACCATCAA1045320.5074235425456282No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGCTGTAGGCACCATCAATTCGT1001320.48606488120555275No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT955550.4638470191706607No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATTCG897280.4355613556187017No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCG785980.38153365091073815No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGCTGTAGGCACCATCAATT776190.37678134876257136No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATTCG700960.3402628921122561No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGCTTCGGACTGTAGGCACCATCAA634980.30823460858457025No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA614410.298249434408085No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGGCTGTAGGCACCATCAATTCG523410.2540758393638381No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA514750.24987206647281418No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAATTCG485990.23591126874234666No Hit
CCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGACTGTAGGCACCATCAAT466090.2262513287271762No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATTC459470.22303782104373757No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA439530.2133584640637125No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCTGTAGGCACCATCAATTCGTA431820.20961584408798564No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG428020.2077712324267973No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATT427220.20738289312970506No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATT423100.2053829457496798No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT420200.20397521579772027No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG396850.19264056256383935No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG394220.19136389712464846No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA390810.1897086008707926No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGTATGCC367830.17855355456181687No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA361750.17560217590391553No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC358790.1741653205046741Illumina Single End Adapter 1 (95% over 22bp)
TCCGATGTAGTGTAACGGCTATCACATCACGTCTGTAGGCACCATCAATT358030.17379639817243642No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGCTGTAGGCACCATCAAT357360.17347116401112164No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT354160.1719178068227525Illumina Single End Adapter 1 (95% over 22bp)
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCG353570.17163140659114698No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATTCG352590.17115569095220892No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA351720.17073337196662106Illumina Single End Adapter 1 (95% over 22bp)
ATGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT348620.16922855719038848No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA346160.1680344138518297No Hit
TCGATGAAACCCTGGTTCGATTCTAGGAGATGGCCTGTAGGCACCATCAA341230.16564127293349856No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATTCGTA339960.16502478429936454No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATTCGTATGCCGT338070.16410733270998404No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAA332830.1615637103140296No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT324660.1575977952424747No Hit
CCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTCG324600.15756866979519277No Hit
ATGCGAAAGCAGTTGAAGACAAGTTCTGTAGGCACCATCAATTCGTATGC324000.15727741532237358No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT317690.15421438911655821No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT317180.15396682281466187No Hit
CGTGGAGACCGGGGTTCGACTCCCCGCTTCGGACTGTAGGCACCATCAAT310950.15094263053855572No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA310630.15078729481971884No Hit
TTGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTC301120.14617091142553434No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCTGTAGGCACCATCAAT298070.14469036785537No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG294610.1430108003954459No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGCTGTAGGCACCATCAAT293680.14255935596257613No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATT278890.1353799332075826No Hit
TGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAAT277030.13447704434184304No Hit
TCGGGGATCAGCGGTTCGTTCCCGCTAGAGACTGTAGGCACCATCAATTC274890.1334382367221212No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC272070.1320693406998709No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC268830.13049656654664718No Hit
ATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCCTGTAGGCACCATCAATT267830.13001114242528183No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA265990.12911796204196957No Hit
AGGAGAAGACTGCGGGTTCGAGTCCCGTACGGACTGTAGGCACCATCAAT265770.1290111687352692No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG265290.12877816515701385No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG264770.12852574461390384No Hit
ACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA263180.12775392026093293Illumina Single End Adapter 1 (95% over 22bp)
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCTGTAGGCACCATCA257930.12520544362376484No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATT256610.1245646837835626No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATTC251390.12203076987003546No Hit
TGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAATTC250080.12139486427104684No Hit
TATGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATT249220.12097739952667264No Hit
TCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTGTAGGCACCATCAAT242090.1175163255413377No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCCTGTAGGCACCATCAATT240220.1166085824343845No Hit
TGGGTGGGTACAAATGGCAGTCTGACAAGTCTGTAGGCACCATCAATTCG235820.11447271630037695No Hit
TCGTGGTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATTCGT230360.11182230059772214No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACTGTAGGCACCATCA224570.10901169493501676No Hit
ATGGAATAATAGAATAGGACGTTTGGTTCTACTGTAGGCACCATCAATTC218380.10600691962376523No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT206830.10040027102199545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)