FastQCFastQC Report
Sun 1 Oct 2023
SRR7471241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66352535
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT710112410.70211409405835No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC43601896.571247051827032No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA27622274.162956245756097No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA15332082.310699960446123No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA12622281.902305616507342No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG11681891.7605793056738526No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC9036361.3618711025886199No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG8690451.3097389572229607No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6597510.9943116717394445Illumina Single End Adapter 1 (95% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT6556020.9880587079302998No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG6302920.9499139708829513No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG5945610.8960637298936657No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG5353730.8068614107961362No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT5029110.7579378843626698No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC4850420.7310074890130422No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT4737140.7139350440793257No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA4659350.7022113021002137No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT4568720.6885524418923858No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC4498500.6779695756914186No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT4353300.6560864630115488No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG4303850.6486338464687145No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT4023850.6064350065901778No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC3796610.5721876338258968No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT3551520.5352500850193591No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT3359130.5062549607185317No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC3147940.47442648574014545No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG3086860.46522111024092755No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT2964910.4468420083724005No Hit
TGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATGC2691750.4056740258680395No Hit
TCCAACGGGGCCTTTCCTTCTGGCTGTAGGCACCATCAATTCGTATGCCG2530960.3814413420677899No Hit
GAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT2376810.3582093736132312No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT2335380.351965452412632No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT2281810.3438919100830134No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCCG1956470.2948598723470023No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1914180.288486340423919No Hit
GTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT1718700.2590255217830034No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT1468540.2213238725543794No Hit
TGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGC1384140.2086039365338491No Hit
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG1286790.19393230416893642No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG1256150.18931454540508513No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG1190350.179397818033629No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC1179290.1777309638584268No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG1162870.1752563033198355No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT1113630.16783533590690997No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGC1076330.16221384759451918No Hit
TGATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTA1053870.15882889779569087No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT1014800.15294065253121075No Hit
CGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG1007490.15183896139009612No Hit
CCGGAGATGGGGTCTTATGGCTGGCTGTAGGCACCATCAATTCGTATGCC937030.14121992475494718No Hit
ATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCCGTCT922100.13896982232856664No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGTA917810.1383232758175705No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCC911680.1373994226445154No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG908230.13687947265315484No Hit
TGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGCCG904880.13637459367603666No Hit
AGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG821820.1238566092463536No Hit
GTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTC820710.12368932098826366No Hit
TTTGTGCCCTCTGCTCCTTGTGGGTAGGGGACTGTAGGCACCATCAATTC791360.11926597830813848No Hit
GATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGC785650.1184054233949012No Hit
GGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCCG774250.11668732777127505No Hit
TGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTATG762050.11484866403371023No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATG753130.11350432956329401No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA742960.11197160741484857No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT721850.10879011630829177No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA717790.10817823313005298No Hit
TATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTA705350.10630339895830655No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT704910.10623708649564029No Hit
TTTGGAAGAGATGTATTTATTAGATAAACTGTAGGCACCATCAATTCGTA680820.10260647916466191No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG674040.10158466439903163No Hit
TTGTGCCCTCTGCTCCTTGTGGGTAGGGGACTGTAGGCACCATCAATTCG668670.10077535093421827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)