FastQCFastQC Report
Sun 1 Oct 2023
SRR7471242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43062906
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA807122418.74286886258907Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT10530382.4453482075733577No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA9427972.189348298974528No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA7394881.7172273510756568No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT6978261.6204805128571678No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT6485671.506092041257039No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT6078241.4114792903200728No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC5469051.2700141509260894No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC5332611.2383302696757157No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGT3707870.8610357136603833No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG3644590.8463409320309223No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC2925590.6793758879161569No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT2444390.5676323841219634No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG2257080.524135551836655No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT2217640.5149768573444625No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG2150220.4993206914554257No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC2146660.49849399387955845No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG1941760.4509124395831531No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA1898080.4407691389893659No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC1695210.393658988085941No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG1689640.3923655314854971No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG1655330.3843981174888662No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATG1605000.3727105644008326No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1589560.3691251119931386Illumina Single End Adapter 1 (95% over 22bp)
TGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATTCGT1554580.36100211165498214No Hit
TCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATTCGTA1548680.35963202297587626No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG1489840.34596829113204763No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATTCGTATGCCG1362430.3163813422159666No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1344030.31210852328451777No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC1308140.30377420418399076No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATGC1269170.2947246523492864No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC1103160.2561740724139704No Hit
GTCTAGTCGGTTATGGCATCTGCTGTAGGCACCATCAATTCGTATGCCGT904330.21000208392810277No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCTGTAGGCACCATCAATTCGTA846490.19657057050446156No Hit
TTGGGTAAAACCATACTGCAGACATCTCTGTAGGCACCATCAATTCGTAT835850.19409976651366723No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG828530.1923999276778952No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA796730.1850153819159348Illumina Single End Adapter 1 (95% over 22bp)
GCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATTCG788000.1829881151076985No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC780080.181148945219814No Hit
TTGGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATTCG732650.17013482555032397No Hit
TTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATGC693490.16104115221578402No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATTCG688170.1598057502203869No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT665580.15455993610835272No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC663710.15412568766260223No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCC646720.15018029670361774No Hit
TCCGTGATAGTTTAATGGTCAGAATGCTGTAGGCACCATCAATTCGTATG636400.1477838026072834No Hit
TGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAATTCG620560.14410546283151443No Hit
GGCGGTTAGTGTAGTGGTTATCATCCCCTGTAGGCACCATCAATTCGTAT601700.13972582342677942No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATTCGTA599600.1392381647443858No Hit
CTGTAGGCACATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA592000.13747330475096128Illumina Single End Adapter 1 (95% over 22bp)
GGGGACACGGGTTCGATTCTCGTACCGCCTGTAGGCACCATCAATTCGTA590570.13714123241009327No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG589460.13688346996368522No Hit
AGAGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTAT585560.13597781812495421No Hit
TGCGGTTAGTGTAGTGGTTATCATCCCCTGTAGGCACCATCAATTCGTAT583360.13546693760054185No Hit
ATGCGAAAGCAGTTGAAGACAAGTTCTGTAGGCACCATCAATTCGTATGC577250.13404808305319663No Hit
AGCATGGGGCCCGGGTTCGATTCCCGGCTCTGTAGGCACCATCAATTCGT571420.13269424966350388No Hit
ATGCGAAAGCAGTTGAAGACAAGTCTGTAGGCACCATCAATTCGTATGCC562310.13057873985559637No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT550270.12778283007653965Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGTACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA546680.12694916594806677Illumina PCR Primer Index 2 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC528570.12274369035847232Illumina Single End Adapter 1 (95% over 22bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG520180.12079537781309975No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG514630.11950656558105947No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC511380.11875185571545031No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGCTGTAGGCACCATCAATT509890.11840585026937105No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT504980.11726565782625073No Hit
TCCGATATAGTGTAACGGCTATCACATCCTGTAGGCACCATCAATTCGTA499480.11598845651521986No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG473140.10987182332748283No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG468020.10868286501612316No Hit
TGCGGGAGGTCCCGGGTTCGAGTCCCGGCCTGTAGGCACCATCAATTCGT463040.10752641728358973No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATTCGT461350.10713396815347297No Hit
AGGTTAGGGGTTCGAGCCCCCTACAGGGCCTGTAGGCACCATCAATTCGT443380.10296100314270476No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA440570.10230846938197807Illumina Single End Adapter 1 (95% over 22bp)
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATTCGTATGC431150.10012097186381244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)