FastQCFastQC Report
Sun 1 Oct 2023
SRR7471243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40374317
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length20-45
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATGTATTTATTAGATAAAAAATCAATG12291733.04444283230847No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCA11876432.9415804111311656No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA8592902.1283084491559325No Hit
AGAGATGTATTTATTAGATAAAAAATCA4803731.1897984552902778No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT4626561.1459165984157702No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG4593071.1376217212541329No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT2601390.6443180202899779No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG2513050.6224377739937991No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACG2510740.621865628092235No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTC2340390.5796729638794881No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA2276230.5637816733840971No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA2176420.5390605121567753No Hit
ATCTTTTCTTCTTAACAGCTTATCACCCCGGAAT1911450.4734321573786623No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1724280.4270734784194615No Hit
TAGAGATGTATTTATTAGATAAAAAATCA1584960.3925663931355173No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC1435180.3554685519509841No Hit
TTTTGGAAGAGATGTATTTATTAGATAAAAAATCA1335440.3307647284782551No Hit
AAGAGATGTATTTATTAGATAAAAAATCA1321400.327287270271346No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT1318280.3265145017809218No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT1318200.3264946872042442No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA1144570.283489625347718No Hit
AAAGAGATGTATTTATTAGATAAAAAATCAATG1119940.2773892125531189No Hit
ATGTATTTATTAGATAAAAAATCAATG1045700.2590012853963573No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC1014910.25137515019758727No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG968690.23992727852213575No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACG925400.22920511571749932No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAATG855930.21199863269513636No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAA851230.21083452631533062No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCC834070.20658429961799726No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAATG822330.20367651049056756No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATT736780.18248729755601809No Hit
ATGCCCTGACGAGTAGGCAGGCGTGGAGGTCA721250.17864079285848972No Hit
ATGGGTGGGTACAAATGGCAGTCTGACAAGT670580.1660907353553498No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT667090.1652263244477919No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTT662820.16416872141762795No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT651220.16129560779938396No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAA643590.15940579254876314No Hit
AGATGTATTTATTAGATAAAAAATCAATG641350.15885098440179185No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA628300.15561873158126738No Hit
AGAGATGTATTTATTAGATAAAAAATC594400.14722230471415776No Hit
ATGTATTTATTAGATAAAAAATCA571460.1415404748518718No Hit
AAAGAGATGTATTTATTAGATAAAAAATCA570970.14141911056972184No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA539830.13370628659798753No Hit
AGAGGTGCACAATCGACCGATCCTG527280.1305978748816977No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAAC526640.13043935826827735No Hit
AAAAGCTCGTAGTTGAACTTTGGGCCCGGTTGGC519870.1287625497169401No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT511490.1266869728099673No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCC475430.11775555237256398No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA475250.1177109695750395No Hit
TTTGGAAGAGATGTATTTATTAGATAAA463010.11467933934337514No Hit
TAGAGATGTATTTATTAGATAAAAAATCAAT457970.11343102101268983No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCAAT456220.11299757714786852No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT455920.11292327248532774No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAAT449710.11138516597073332No Hit
AGAGATGTATTTATTAGATAAAAAATCAA445660.11038205302643263No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCAATG442970.10971578788565017No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT420280.10409587857548154No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT411580.10194104336179854No Hit
TAAGAGATGTATTTATTAGATAAAAAATCA409880.10151998360740072No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTC408010.10105681787756311No Hit

[OK]Adapter Content

Adapter graph