FastQCFastQC Report
Sun 1 Oct 2023
SRR7471244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46056743
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT26972465.856354193347975No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC21562144.681646724346096No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9822322.13265623233497Illumina Single End Adapter 1 (95% over 22bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA9019791.9584081314651365No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC7307071.5865364166111353No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT7187191.5605076546554757No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT5849661.2700984956752153No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA4425720.9609276973840725No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT4181180.9078323232713178No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCCG3538600.7683131219244053No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC3103290.6737971028476764No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG2971930.6452757634207873No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG2843580.61740796564794No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC2602700.5651072634467444No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC2556640.5551065562755925No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG2543490.5522513826042801No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC2433960.5284698485952426No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG2149760.466763357539199No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA2136330.46384738929541763No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG1994550.43306362327878895No Hit
TCCAACGGGGCCTTTCCTTCTGGCTGTAGGCACCATCAATTCGTATGCCG1983560.4306774363093803No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1909190.41452996361466554No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT1767640.3837961359968507No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG1645420.35725930511412846No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT1633700.3547146180093542No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG1469360.3190325464395083No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT1430660.31062986803039894No Hit
AGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCCGTCT1378580.29932207755116336No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC1324990.28768643062754135No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG1232150.26752868738460295No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC1164200.2527751473872132No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCC1160570.25198698917984713No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATTCGTATG1090940.23686868174764333No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA1090820.23684262693087088No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGC1004870.21818086441761633No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA968410.21026454258825902No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGT944490.20507094911162085No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT934040.20280200881768823No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTAT916370.19896543704794756No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC913870.1984226283651886No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT886730.19252989730515682No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTATGCC857540.18619206312526268No Hit
TCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCCG852980.18520198008791025No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATTCGTATGC845510.18358006774382635No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG826200.17938741347819578No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA797930.17324933289355698No Hit
ATGTATTTATTAGATAAAAAATCAATCTGTAGGCACCATCAATTCGTATG733760.15931651962449886No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT728960.15827432695360155No Hit
ATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGCCG636090.13811007000647005No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATTCGT620930.13481847815421946No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATTCG618830.13436251886070188No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTATG541310.11753110722571068No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT539800.11720325078132425No Hit
TAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCCGTC501840.10896124374231153No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGT493620.10717648879339992No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCGTAT488500.10606481661110947No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATTCGTATGC484560.10520935012708128No Hit
AGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATGCCGTC476780.10352012950633525No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT476590.10347887604644558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)