FastQCFastQC Report
Sun 1 Oct 2023
SRR7471245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7471245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38654546
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT15322883.9640563880895145No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCC12318723.1868748374382667No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTA5009121.2958682789858662No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCG4325111.1189136718873893No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATG4154271.0747170591526285No Hit
TTTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATG3558840.9206782560581619No Hit
TTGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGC3403620.8805225651854764No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT3383150.8752269396722445No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGCCG2832690.7328219557927287No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCG2767410.715933903349945No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT2600130.6726582689653113No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATTCGTATGC2584270.6685552586751375No Hit
AGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTA2216670.573456482970981No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATG1968750.5093191367452615No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTATGC1885060.4876683844637575No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGT1855370.4799875285044093No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTATGCC1423960.3683809919795721No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA1350390.3493483017495536No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATTCGTAT1311230.3392175398981533No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATT1291560.33412887581191614No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTA1256270.32499928986360366No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT1247790.3228054987374577No Hit
TTACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATTCGTAT1192850.3085924227385829No Hit
TGGAAGAGATGTATTTATTAGATACTGTAGGCACCATCAATTCGTATGCC1186040.30683066359128885No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCG1154390.2986427521358031No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT1103850.28556796398540035No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATTCGTA1012280.2618786416479966No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATTCGT991470.25649505752829177No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTC971240.2512615204431582No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTATGCCGT950480.24589087141264057No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA927700.23999764477896082No Hit
TCCAACGGGGCCTTTCCTTCTGGCTGTAGGCACCATCAATTCGTATGCCG916690.23714933813994346No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT877000.22688146434315903No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAA860520.22261805894706407No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATG839880.21727845413059568No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG831530.21511829423633638No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTAT826060.21370319547925876No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGCTGTAGGCACCATCAATTC820750.2123294890075801No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATTCGTATGC749130.19380126725586172No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTC747200.19330197281323652No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT738240.19098400483089364No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATTCGTAT725100.1875846633925024No Hit
TTTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTAT710830.18389298893848086No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGT709060.18343508678125467No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATTCGTATGCC704030.18213381680902424No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA701380.1814482570821036No Hit
TAGAGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGT671250.17365357233790818No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA657310.17004726947252208Illumina Single End Adapter 1 (95% over 22bp)
TTGGAAGAGATGTATTTATTAGATAACTGTAGGCACCATCAATTCGTATG651600.16857008228734596No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGTATGC639540.16545013877539785No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATTC553540.14320178537344613No Hit
TGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATTCGTATGC548440.14188240627635362No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAATT543320.14055785314358626No Hit
TGTGCCCTCTGCTCCTTGTGGGTAGGGGACTGTAGGCACCATCAATTCGT525280.13589087296485128No Hit
TTTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGC465760.12049294279643072No Hit
GTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT463040.11978927394464807No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCAATTCG459080.11876481488102332No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATTCGTATGCCG444240.11492568041026792No Hit
TTGGAAGAGATGTATTTATTAGATCTGTAGGCACCATCAATTCGTATGCC440790.11403315925635242No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGT430260.11130902947353204No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGT423660.10960159770082412No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATTCGTA421790.10911782536522355No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTCTGTAGGCACCATCAATTC420240.10871683760042092No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATTCG413170.1068878159893535No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATTCGTAT412460.10670413772289553No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT410670.10624106152999442No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATTCGTAT410150.10610653660244773No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATTCG409710.10599270781760053No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATTCGTAT402760.10419473042058236No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATTCGT400790.10368508790660742No Hit
CCGGAGATGGGGTCTTATGGCTGCTGTAGGCACCATCAATTCGTATGCCG395160.10222859686413081No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT394940.10217168247170721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)